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Titolo:
Detection of the 20-kDa virulence-associated antigen of Rhodococcus equi in malakoplakia-like lesion in pleural tissue obtained from an AIDS patient
Autore:
Caterino-de-Araujo, A; de los Santos-Fortuna, E; Zandona-Meleiro, MC; Calore, EE; Calore, NMP;
Indirizzi:
Inst Adolfo Lutz, Dept Immunol, BR-01246902 Sao Paulo, Brazil Inst Adolfo Lutz Sao Paulo Brazil BR-01246902 BC246902 Sao Paulo, Brazil Emilio Ribas Inst, Pathol Sect, Sao Paulo, Brazil Emilio Ribas Inst Sao Paulo Brazil Inst, Pathol Sect, Sao Paulo, Brazil
Titolo Testata:
PATHOLOGY RESEARCH AND PRACTICE
fascicolo: 5, volume: 196, anno: 2000,
pagine: 321 - 327
SICI:
0344-0338(2000)196:5<321:DOT2VA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FINE-NEEDLE ASPIRATION; ACQUIRED-IMMUNODEFICIENCY-SYNDROME; PULMONARY MALACOPLAKIA; 17-KILODALTON ANTIGENS; IDENTIFICATION; INFECTION; ANTIBODY; 15-KILODALTON; PATHOGEN; PLASMIDS;
Keywords:
Rhodococcus equi; malakoplakia; AIDS; virulence-associated antigen of 20-kDa immunohistochemistry; macrophages;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Caterino-de-Araujo, A Inst Adolfo Lutz, Dept Immunol, Av Dr Arnaldo 355,11Andar, BR-01246902 Sao Paulo, Brazil Inst Adolfo Lutz Av Dr Arnaldo 355,11Andar Sao Paulo Brazil BR-01246902 BC
Citazione:
A. Caterino-de-Araujo et al., "Detection of the 20-kDa virulence-associated antigen of Rhodococcus equi in malakoplakia-like lesion in pleural tissue obtained from an AIDS patient", PATH RES PR, 196(5), 2000, pp. 321-327

Abstract

A malakoplakia-like lesion was detected in a pleural biopsy from an AIDS patient presenting clinical and radiologic features of pneumonia. Cultures of bronchoalveolar lavage and pleural fluid evidenced Rhodococcus equi as the causative agent of pleuro-pulmonary infection. Immunochemical characterization of the R. equi isolate showed the presence of a strain similar to theATCC 33704 reference strain presenting the capsular antigen of serotype 4,and the intermediate virulence-associated antigen of 20-kDa. Histopathology of the patient's pleural biopsy showed plaques of macrophages interspersed with lymphocytes, and intracytoplasmic cocci and bacilli in macrophages, which were variably acid-fast positive. Immunohistochemistry of cocci, bacilli and their degradation products resulted strongly positive when stained with a mouse monoclonal antibody (MAb) produced against the 20-kDa antigen. This finding could have important implications for the pathogenicity of R.equi for human beings, since we do not know yet all the factors involved in the formation of malakoplakia. Indeed, the results obtained in the present study, taken together with the results obtained for pigs inoculated with R. equi strains of intermediate virulence (Madarame ct al. 1998), raise thepossibility that most strains presenting the 20-kDa antigen may be capableof inducing malakoplakia. If this hyphothesis is confirmed by immunohistochemical analysis of human pulmonary malakoplakia cases due to R. equi, the detection of this antigen may be extremely helpful in the diagnosis and treatment of such patients. This is the first report of R. equi infection in human beings that suggests a relationship between pleural malakoplakia and the virulence-associated antigen of 20-kDa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 03:04:07