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Titolo:
An alternatively spliced transcript of the rat nociceptin receptor ORL1 gene encodes a truncated receptor
Autore:
Xie, GX; Ito, E; Maruyama, K; Pietruck, C; Sharma, M; Yu, LC; Palmer, PP;
Indirizzi:
Univ Calif San Francisco, Dept Anesthesia, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Tokyo Med & Dent Univ, Sch Med, Dept Hyg & Oncol, Tokyo, Japan Tokyo Med &Dent Univ Tokyo Japan h Med, Dept Hyg & Oncol, Tokyo, Japan
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1, volume: 77, anno: 2000,
pagine: 1 - 9
SICI:
0169-328X(20000414)77:1<1:AASTOT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; ORPHANIN FQ RECEPTOR; KAPPA-OPIOID RECEPTORS; MESSENGER-RNA; MICE LACKING; KNOCKOUT MICE; DYNORPHIN-A; EXPRESSION; CLONING; MEMBER;
Keywords:
alternative splicing; receptor; opioid; nociceptin; orphanin FQ; genomic sequence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Palmer, PP Univ Calif San Francisco, Dept Anesthesia, POB 0464,Rm S-455,513 ParnassusAve, San Francisco, CA 94143 USA Univ Calif San Francisco POB 0464,Rm S-455,513 Parnassus Ave San Francisco CA USA 94143
Citazione:
G.X. Xie et al., "An alternatively spliced transcript of the rat nociceptin receptor ORL1 gene encodes a truncated receptor", MOL BRAIN R, 77(1), 2000, pp. 1-9

Abstract

Opioid receptor-like protein ORL1, the receptor for the neuropeptide nociceptin (also named orphanin FQ), has two alternatively spliced isoforms in the rat. This alternative splicing event is generated by retaining of intron3, 81 bases in length, in the mRNA region encoding the second extracellular loop of ORL1. A full-length rat ORL1 receptor has 367 amino acid residues. However, as revealed by sequencing of rat ORL1 genomic DNA and cDNA, the insertion of the unspliced intron 3 brings in an in-frame stop codon and, therefore, creates a truncated open-reading frame encoding only the N-terminal half of ORL1 (from the N-terminus to an alternate extracellular tail C-terminal to the fourth transmembrane domain). The two alternatively spliced transcripts are differentially expressed in tissues. In transfected mammalian cells, the full-length ORL1 displays high-affinity and selective bindingfor nociceptin, and inhibits the production of cyclic AMP. In contrast, the truncated ORL1 binds nociceptin and other opioid peptides very poorly andnon-selectively (affinity in micromolar range), and it does not mediate any inhibitory effects on cyclic AMP production. Apparently, this truncated ORL1 does not function as a receptor for nociceptin or other ligands tested. Such alternative splicing to create a truncated ORL1 receptor might be an endogenous mechanism to negatively regulate nociceptin/ORL1 functions. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:27:26