Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Assessment of thymic output in adults after haematopoietic stem-cell transplantation and prediction of T-cell reconstitution
Autore:
Douek, DC; Vescio, RA; Betts, MR; Brenchley, JM; Hill, BJ; Zhang, L; Berenson, JR; Collins, RH; Koup, RA;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 r, Dept Internal Med, Dallas, TX 75390 USA Univ Calif Los Angeles, W Los Angeles Vet Affairs Med Ctr, Dept Hematol Oncol, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA tol Oncol, Los Angeles, CA USA
Titolo Testata:
LANCET
fascicolo: 9218, volume: 355, anno: 2000,
pagine: 1875 - 1881
SICI:
0140-6736(20000527)355:9218<1875:AOTOIA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; INTENSIVE CHEMOTHERAPY; IMMUNE RECONSTITUTION; AGE; EXPANSION; MEMORY; THYMOPOIESIS; REGENERATION; PROGENITORS; GENERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Douek, DC Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 ternal Med, Dallas, TX 75390 USA
Citazione:
D.C. Douek et al., "Assessment of thymic output in adults after haematopoietic stem-cell transplantation and prediction of T-cell reconstitution", LANCET, 355(9218), 2000, pp. 1875-1881

Abstract

Background The potential benefits of haematopoietic stem-cell transplantation are tempered by the depletion of T-cells accompanying this procedure. We used a new technique which quantifies the excisional DNA products of T-cell-receptor (TCR) gene rearrangement to measure thymic output directly in patients with multiple myeloma, and thus assessed the contribution of the thymus to immune recovery after transplantation. Methods We studied 40 patients, 34-66 years of age, who had been randomly assigned myeloablative chemotherapy and autologous peripheral-blood haematopoietic stem-cell transplantation with unmanipulated grafts or grafts enriched for CD34 stem cells. CD4 and CD8 T-cell counts were measured, thymic output was estimated serially until 2 years after transplantation, and percentages of naive T-cells were measured. Findings The production of substantial numbers of new naive T cells by thethymus could be detected by 100 days post-transplant; there was a significant inverse relation between age and recovery of new T cells. In the CD34-unselected group, numbers of TCR-rearrangement excision circles returned to baseline after 2 years, whereas in the CD34-selected group, numbers at 2 years were significantly higher than both baseline numbers (p=0.004), and 2-year numbers in the unselected group (p=0.046). Increased thymic output correlated with, and was predictive of, increased naive T-cell numbers and broader T-cell-receptor repertoires. Interpretation Our results provide evidence that the adult thymus contributes more substantially to immune reconstitution after haematopoietic stem-cell transplantation than was previously thought, and therefore could be a target for therapeutic intervention.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:09:51