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Titolo: Effect of smear layer and direction of dentinal tubules on osteoblast adhesion to human dentin tissue
Autore: Zhu, Q; Haglund, R; Chiou, JL; Dean, JW;
- Indirizzi:
- Univ Connecticut, Ctr Hlth, Dept Endodontol, Farmington, CT 06030 USA UnivConnecticut Farmington CT USA 06030 dontol, Farmington, CT 06030 USA Univ Connecticut, Ctr Hlth, Sch Dent Med, Dept Periodontol, Farmington, CT06030 USA Univ Connecticut Farmington CT USA 06030 odontol, Farmington, CT06030 USA
- Titolo Testata:
- JOURNAL OF ENDODONTICS
fascicolo: 6,
volume: 26,
anno: 2000,
pagine: 318 - 320
- SICI:
- 0099-2399(200006)26:6<318:EOSLAD>2.0.ZU;2-2
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- ROOT ENDS; PERIRADICULAR SURGERY; PERMEABILITY;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Clinical Medicine
- Citazioni:
- 12
- Recensione:
- Indirizzi per estratti:
- Indirizzo: Zhu, Q Univ Connecticut, Ctr Hlth, Dept Endodontol, Farmington, CT 06030 USA Univ Connecticut Farmington CT USA 06030 Farmington, CT 06030 USA
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- Citazione:
- Q. Zhu et al., "Effect of smear layer and direction of dentinal tubules on osteoblast adhesion to human dentin tissue", J ENDODONT, 26(6), 2000, pp. 318-320
Abstract
The purpose of this study was to investigate the effect of smear layer anddirection of dentinal tubules on osteoblast adhesion to human dentin tissue in vitro, Dentin disks were made from human premolars extracted for orthodontic reasons. Dentin disks were cut either perpendicularly to the long axis of the tooth or at 45 degrees to the long axis of tooth. The smear layerwas removed by 34% phosphoric acid gel from half of the dentin disk surface. Human osteoblast-like Saos-2 cells were grown in RPMI medium with 10% fetal bovine serum and 1% antibiotic/antimycotic cocktail under standard cellculture conditions. Cells were seeded into Nunc four-well culture plates at 1.5 x 10(5) cells per well with dentin disks in the bottom of each well. After 1 day in culture the dentin disks along with cells grown on their surface were examined with a scanning electron microscopy. Osteoblasts attached and spread on the dentin surface and formed a monolayer in the presence and absence of a smear layer. Cells spread over the dentinal tubules despitetheir direction, These results suggest that cell adhesion and spreading ofosteoblasts is not influenced either by the existence of a smear layer or the direction of the dentinal tubules on the dentin surface.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/21 alle ore 10:08:47