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Titolo:
Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids
Autore:
Kojima, Y; Fukumoto, S; Furukawa, K; Okajima, T; Wiels, J; Yokoyama, K; Suzuki, Y; Urano, T; Ohta, M; Furukawa, K;
Indirizzi:
Nagoya Univ, Sch Med, Dept Biochem 2, Nagoya, Aichi 4660065, Japan Nagoya Univ Nagoya Aichi Japan 4660065 em 2, Nagoya, Aichi 4660065, Japan Nagoya Univ, Sch Med, Dept Bacteriol, Nagoya, Aichi 4660065, Japan Nagoya Univ Nagoya Aichi Japan 4660065 riol, Nagoya, Aichi 4660065, Japan Nagasaki Univ, Sch Dent, Dept Pediat Dent, Nagasaki 8528588, Japan Nagasaki Univ Nagasaki Japan 8528588 ediat Dent, Nagasaki 8528588, Japan Univ Shizuoka, Sch Pharmaceut Sci, Dept Biochem, Shizuoka 4228526, Japan Univ Shizuoka Shizuoka Japan 4228526 pt Biochem, Shizuoka 4228526, Japan Inst Gustave Roussy, CNRS, UMR 1598, F-94805 Villejuif, France Inst Gustave Roussy Villejuif France F-94805 , F-94805 Villejuif, France
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 20, volume: 275, anno: 2000,
pagine: 15152 - 15156
SICI:
0021-9258(20000519)275:20<15152:MCOGSA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOMA-ASSOCIATED ANTIGEN; CENTER B-CELLS; BURKITTS-LYMPHOMA; MONOCLONAL-ANTIBODY; UDP-GALACTOSE; EUKARYOTIC RIBOSOMES; GLYCOLIPID ANTIGEN; APOPTOSIS; BIOSYNTHESIS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Nagoya Univ, Sch Med, Dept Biochem 2, Nagoya, Aichi 4660065, Japan Nagoya Univ Nagoya Aichi Japan 4660065 , Aichi 4660065, Japan
Citazione:
Y. Kojima et al., "Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids", J BIOL CHEM, 275(20), 2000, pp. 15152-15156

Abstract

The expression cloning of a cDNA for globotriaosylceramide (Gb3)/CD77 synthase (alpha 1,4-galactosyltransferase) was achieved using an anti-Gb3 antibody and mouse L cells as a recipient cell line for the transfection. The isolated cDNA clone designated pVTR1 predicted a type II membrane protein with 19 amino acids of cytoplasmic domain, 26 amino acids of transmembrane region, and a catalytic domain with 308 amino acids. Introduction of the cDNA clone into L cells resulted in the neosynthesis of Gb3/CD77, and the extracts of the transfectant cells showed alpha 1,4-galactosyltransferase activity only on lactosylceramide and galactosylceramide, In Northern blotting, a 2.3-kilobase mRNA was strongly expressed in heart, kidney, spleen, and placenta and weakly in colon, small intestine, and brain. Transfection of the cDNA into L cells resulted in the constitution of sensitivity to the apoptosis with Shiga-like toxins (verotoxins). Since Gh3/CD77 synthase initiates the synthesis of globe series glycolipids, the isolation of this cDNA will make possible further investigations into the function of its important series of glycolipids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:09:59