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Titolo:
High-performance liquid chromatographic method for determination of biogenic amines in feedstuffs, complete feeds, and animal tissues
Autore:
Salazar, MT; Smith, TK; Harris, A;
Indirizzi:
Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 ltry Sci, Guelph, ON N1G 2W1, Canada Univ Autonoma Nuevo Leon, Fac Ciencias Biol, Programa Maricultura, Monterey, Nuevo Leon, Mexico Univ Autonoma Nuevo Leon Monterey Nuevo Leon Mexico, Nuevo Leon, Mexico
Titolo Testata:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
fascicolo: 5, volume: 48, anno: 2000,
pagine: 1708 - 1712
SICI:
0021-8561(200005)48:5<1708:HLCMFD>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FISH-MEAL; POLYAMINES; SEPARATION; GROWTH; FOODS;
Keywords:
high-performance liquid chromatographic; biogenic amines; feedstuffs; complete feeds; animal tissues;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, TK Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, CanadaUniv Guelph Guelph ON Canada N1G 2W1 Guelph, ON N1G 2W1, Canada
Citazione:
M.T. Salazar et al., "High-performance liquid chromatographic method for determination of biogenic amines in feedstuffs, complete feeds, and animal tissues", J AGR FOOD, 48(5), 2000, pp. 1708-1712

Abstract

Numerous methods to analyze biogenic amines in biological materials have been described. A versatile and rapid methodology to analyze these compoundsin feedstuffs, complete feeds, and animal tissues, however, has not been reported. The current method was developed to address this need. Biogenic amines in feedstuffs, complete animal feeds, and animal tissues were extracted with 10% trichloroacetic acid, reacted with O-phthaladehyde using high-performance liquid chromatographic employing a cation exchange column. Detection limits were 50 pmol/mL for tyramine, histamine, putrescine, and spermine; 40 pmol/mL for cadaverine; and 25 pmol/mL for spermidine. Extraction efficiency of biogenic amines in feedstuffs, duodenum, liver, ileum + jejunum,and whale shrimp and shrimp hepatopancreas ranged between 99-105, 93-135, 80-85, 65-102, 88-98, and 88-97%, respectively. It can be concluded that the current method can be applied to individual feedstuffs, complete feeds, and animal tissues for the rapid and accurate determination of concentrationof biogenic amines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:24:30