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Titolo:
Characterization of hemolysin of Moraxella bovis using a hemolysis-neutralizing monoclonal antibody
Autore:
Billson, FM; Harbour, C; Michalski, WP; Tennent, JM; Egerton, JR; Hodgson, JL;
Indirizzi:
Univ Sydney, Dept Vet Clin Sci, Camden, NSW 2570, Australia Univ Sydney Camden NSW Australia 2570 in Sci, Camden, NSW 2570, Australia Univ Sydney, Dept Infect Dis, Sydney, NSW 2000, Australia Univ Sydney Sydney NSW Australia 2000 ct Dis, Sydney, NSW 2000, Australia CSIRO, Div Anim Hlth, Australian Anim Hlth Lab, Geelong, Vic 3220, Australia CSIRO Geelong Vic Australia 3220 m Hlth Lab, Geelong, Vic 3220, Australia
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 6, volume: 68, anno: 2000,
pagine: 3469 - 3474
SICI:
0019-9567(200006)68:6<3469:COHOMB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI HEMOLYSIN; ALPHA-HEMOLYSIN; ERYTHROCYTE-MEMBRANES; PROTECTIVE EFFICACY; CELL-MEMBRANES; KERATOCONJUNCTIVITIS; BINDING; LEUKOTOXIN; CALCIUM; HLYA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Hodgson, JL Univ Narellan, Vet Ctr, Camden PMB 4, Narellan, NSW 2567, Australia Univ Narellan Camden PMB 4 Narellan NSW Australia 2567 stralia
Citazione:
F.M. Billson et al., "Characterization of hemolysin of Moraxella bovis using a hemolysis-neutralizing monoclonal antibody", INFEC IMMUN, 68(6), 2000, pp. 3469-3474

Abstract

A concentrated bacterial culture supernatant from the hemolytic Moraxella bovis strain UQV 148NF was used to immunize mice and generate monoclonal antibodies (MAbs), One, MAb G3/D7, neutralized the hemolytic activity of M. bovis and recognized a 94-kDa protein by Western blot analysis in hemolytic M. bovis strains representing each of the different fimbrial serogroups, Exposure of corneal epithelial cells to ill, bovis concentrated culture supernatants demonstrated a role for an exotoxin in the pathogenesis of infectious bovine keratoconjunctivitis, while neutralization of hemolytic and cytotoxic activities by MAb G3/D7 implies that these activities are related or have common epitopes, The action of M. bovis hemolysin was further characterized in sheep erythrocyte preparations with a binding step and Ca2+ required for lysis to proceed, similar to the RTX family of bacterial exotoxins. Neutralization of lytic activity in vitro is evidence for the presence of M.bovis antigens, which may be capable of protecting cattle from the development of infectious bovine keratoconjunctivitis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:43:41