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Titolo:
Urate oxidase electrode based on dissolved oxygen probe for urine uric acid determination
Autore:
Dinckaya, E; Akyilmaz, E; Akgol, S;
Indirizzi:
Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bormova Izmir, Turkey Ege Univ Bormova Izmir Turkey TR-35100 m, TR-35100 Bormova Izmir, Turkey
Titolo Testata:
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
fascicolo: 1, volume: 37, anno: 2000,
pagine: 67 - 70
SICI:
0301-1208(200002)37:1<67:UOEBOD>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; CELLULOSE-ACETATE; ENZYME ELECTRODE; BIOSENSOR; ALLANTOIN; OXALATE; CARBON; PLASMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Dinckaya, E Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bormova Izmir, Turkey Ege Univ Bormova Izmir Turkey TR-35100 Bormova Izmir, Turkey
Citazione:
E. Dinckaya et al., "Urate oxidase electrode based on dissolved oxygen probe for urine uric acid determination", I J BIOCH B, 37(1), 2000, pp. 67-70

Abstract

A biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC 1.7.3.3) in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35 degrees C. The enzyme electrode response depends linearly on uric acid concentration between 5-40 mu M with a response time of 5 min. Theenzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed.

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Documento generato il 14/08/20 alle ore 08:20:41