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Titolo:
Cripto-1-induced increase in vimentin expression is associated with enhanced migration of human Caski cervical carcinoma cells
Autore:
Ebert, AD; Wechselberger, C; Nees, M; Clair, T; Schaller, G; Martinez-Lacaci, I; Wallace-Jones, B; Bianco, C; Weitzel, HK; Salomon, DS;
Indirizzi:
NCI, Tumor Immunol & Biol Lab, NIH, Growth Factors Sect, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 IH, Growth Factors Sect, Bethesda, MD 20892 USA NCI, Cellular Carcinogenesis & Tumor Promot Lab, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 & Tumor Promot Lab, NIH, Bethesda, MD 20892 USA NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA Free Univ Berlin, Med Ctr Benjamin Franklin, Dept Obstet & Gynecol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 Gynecol, D-12200 Berlin, Germany
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 257, anno: 2000,
pagine: 223 - 229
SICI:
0014-4827(20000525)257:1<223:CIIVEI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATIN INTERMEDIATE FILAMENTS; MAMMARY EPITHELIAL-CELLS; EPITHELIOMESENCHYMAL TRANSFORMATION; PHOSPHATIDYLINOSITOL 3-KINASE; EXPERIMENTAL COEXPRESSION; TYROSINE PHOSPHORYLATION; CANCER CELLS; MOUSE EMBRYO; CRIPTO; GROWTH;
Keywords:
Cripto-1; vimentin; cDNA gene expression array; migration; cervical carcinoma; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Salomon, DS NCI, Tumor Immunol & Biol Lab, NIH, Growth Factors Sect, Bldg 10,Room 5B39,10 Ctr Dr MSC-1750, Bethesda, MD 20892 USA NCI Bldg 10,Room 5B39,10 Ctr Dr MSC-1750 Bethesda MD USA 20892
Citazione:
A.D. Ebert et al., "Cripto-1-induced increase in vimentin expression is associated with enhanced migration of human Caski cervical carcinoma cells", EXP CELL RE, 257(1), 2000, pp. 223-229

Abstract

Cripto-1 (CR-1), a member of the EGF-CFC peptide family, plays an essential role during mesoderm formation in vertebrates as well as in cancer development, Using cDNA gene expression array, Western blot, and indirect immunofluorescence, an increase in vimentin expression was demonstrated in CR-1-transfected human Caski cervical carcinoma cells compared to control vector-transfected cells, In parental Caski cells, recombinant CR-1 induced a dose-dependent increase of vimentin protein expression within 24 h, Since vimentin expression has been demonstrated to correlate with a more aggressive phenotype in human cervical cancer, the migration capacity of CR-1-transfectedor CR-1-treated Caski cells was studied in the Boyden chamber assay, Compared to the vector-transfected or untreated Caski cells, CR-1-transfected cells or cells treated with recombinant CR-1 exhibit enhanced migration, boththrough collagen- and through gelatin-coated membranes, Additionally, CR-1can function as a chemoattractant for Caski cells, These findings are of biological significance since CR-1 is overexpressed in several types of human carcinomas, The present data demonstrate that CR-1 can increase vimentin expression and modulate migration in human cervical carcinoma cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 21:36:39