Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Late endocytic compartments are major sites of annexin VI localization in NRK fibroblasts and polarized WIF-B hepatoma cells
Autore:
Pons, M; Ihrke, G; Koch, S; Biermer, M; Pol, A; Grewal, T; Jackle, S; Enrich, C;
Indirizzi:
Univ Barcelona, Fac Med, IDIBAPS, Dept Biol Cellular, E-08036 Barcelona, Spain Univ Barcelona Barcelona Spain E-08036 ellular, E-08036 Barcelona, Spain Univ Cambridge, Inst Med Res, Dept Clin Biochem, Cambridge CB2 2XY, England Univ Cambridge Cambridge England CB2 2XY hem, Cambridge CB2 2XY, England Univ Cambridge, Wellcome Trust Ctr Study Mol Mechanisms Dis, Inst Med Res,Cambridge CB2 2XY, England Univ Cambridge Cambridge England CB2 2XY Res,Cambridge CB2 2XY, England Univ Hamburg, Hosp Eppendorf, Kernklin & Poliklin, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 Poliklin, D-20246 Hamburg, Germany
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 257, anno: 2000,
pagine: 33 - 47
SICI:
0014-4827(20000525)257:1<33:LECAMS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MANNOSE 6-PHOSPHATE RECEPTOR; PLASMA-MEMBRANE PROTEINS; RAT-LIVER; MULTIVESICULAR ENDOSOMES; PRELYSOSOMAL COMPARTMENT; INTRACELLULAR-TRANSPORT; MONOCLONAL-ANTIBODY; LYSOSOMAL MEMBRANE; VESICLES; CALCIUM;
Keywords:
annexins; endosome; lysosome; prelysosomal compartment; membrane fusion; subapical;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Enrich, C Univ Barcelona, Fac Med, IDIBAPS, Dept Biol Cellular, Casanova 143, E-08036 Barcelona, Spain Univ Barcelona Casanova 143 Barcelona Spain E-08036 lona, Spain
Citazione:
M. Pons et al., "Late endocytic compartments are major sites of annexin VI localization in NRK fibroblasts and polarized WIF-B hepatoma cells", EXP CELL RE, 257(1), 2000, pp. 33-47

Abstract

Annexin VI is an abundant calcium- and phospholipid-binding protein whose intracellular distribution and function are still controversial. Using a highly specific antibody, we have studied the distribution of annexin VI in NRK fibroblasts and the polarized hepatic cell line WIF-B by confocal microscopy. In NRK cells, annexin VI was almost exclusively found associated withendocytic compartments, which were defined by their ability to receive fluid-phase marker internalized from the cell surface. However, extensive colocalization of annexin VI and the endocytic marker was only observed after about 45 min, indicating that annexin VI was primarily in late endocytic compartments or (pre)lysosomes. Consistent with this, annexin VI was predominantly seen on structures that contained the lysosomal protein lgp120, although not on dense core lysosomes by electron microscopy, Two major populations of annexin VI-containing structures were present in polarized WIF-B hepatocytes, One correlated to lgp120-positive (pre)lysosomes and was still observed after treatment with brefeldin A (BFA), while the other appeared to bepartially associated with Golgi membranes and was BFA-sensitive, The striking association with prelysosomal compartments in NRK and WIF-B cells suggests that annexin VI could play a role in fusion events in the late endocytic pathway, possibly by acting as a tether between membranes. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:16:15