Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Lactacystin activates FLICE (caspase 8) protease and induces apoptosis in Fas-resistant adult T-cell leukemia cell lines
Autore:
Yamada, Y; Sugahara, K; Tsuruda, K; Nohda, K; Mori, N; Hata, T; Maeda, T; Hayashibara, T; Joh, T; Honda, M; Tawara, M; Tomonaga, M; Miyazaki, Y; Kamihira, S;
Indirizzi:
Nagasaki Univ, Sch Med, Dept Lab Med, Nagasaki 8528501, Japan Nagasaki Univ Nagasaki Japan 8528501 pt Lab Med, Nagasaki 8528501, Japan Nagasaki Univ, Dept Prevent Med & AIDS Res, Res Field Pathogenesis & Clin Sci, Nagasaki 852, Japan Nagasaki Univ Nagasaki Japan 852 genesis & Clin Sci, Nagasaki 852, Japan Nagasaki Univ, Sch Med, Dept Hematol, Nagasaki 852, Japan Nagasaki Univ Nagasaki Japan 852 Med, Dept Hematol, Nagasaki 852, Japan
Titolo Testata:
EUROPEAN JOURNAL OF HAEMATOLOGY
fascicolo: 5, volume: 64, anno: 2000,
pagine: 315 - 322
SICI:
0902-4441(200005)64:5<315:LAF(8P>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED APOPTOSIS; MICROBIAL METABOLITE; MONOCLONAL-ANTIBODY; THYMOCYTE APOPTOSIS; NEUROBLASTOMA-CELLS; DEATH DOMAIN; U937 CELLS; ATL CELLS; GENE; INVOLVEMENT;
Keywords:
lactacystin; ATL; apoptosis; caspase 8; FLICE; proteasome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Yamada, Y Nagasaki Univ, Sch Med, Dept Lab Med, 1-7-1 Sakamoto, Nagasaki 8528501, Japan Nagasaki Univ 1-7-1 Sakamoto Nagasaki Japan 8528501 8501, Japan
Citazione:
Y. Yamada et al., "Lactacystin activates FLICE (caspase 8) protease and induces apoptosis in Fas-resistant adult T-cell leukemia cell lines", EUR J HAEMA, 64(5), 2000, pp. 315-322

Abstract

Lactacystin (LC) is a specific inhibitor of the proteasome, and has recently been shown to induce apoptosis in certain cell lines. In the present study, we established Fas-resistant adult T-cell leukemia (ATL) cell subclonesRSO4 and RST1 from their parental Fas-sensitive cell lines SO4 and ST1, and examined whether LC can overcome Fas resistance. LC completely inhibited proteasome function as determined by a peptidyl-MCA substrate (LLVY-MCA andLLE-MCA), and induced apoptosis in these cell lines irrespective of Fas sensitivity at low concentrations (similar to 10 mu M). LC induced the activation of caspase 3 (CPP32/Yama) and caspase 6 proteases in an identical manner to Fas-mediated apoptosis. Moreover, LC induced the activation of caspase 8 (FLICE) protease, which is the initiator of the Fas-mediated apoptotic cascade. Synthesized proteasome inhibitory peptide MG-115 (ZLLnV-CHO) also induced apoptosis in these cell lines; These results indicated that proteasome inhibitors overcome Fas-resistance by bypassing the proximal part of the Fas signal. Inhibition of the proteasome function may be a new strategy for the treatment of ATL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:35:06