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Titolo:
Survival of Salmonella species in river water
Autore:
Domingo, JWS; Harmon, S; Bennett, J;
Indirizzi:
US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA US EPA CincinnatiOH USA 45268 Exposure Res Lab, Cincinnati, OH 45268 USA Natl Risk Management Res Lab, Cincinnati, OH 45268 USA Natl Risk Management Res Lab Cincinnati OH USA 45268 innati, OH 45268 USA
Titolo Testata:
CURRENT MICROBIOLOGY
fascicolo: 6, volume: 40, anno: 2000,
pagine: 409 - 417
SICI:
0343-8651(200006)40:6<409:SOSSIR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONCULTURABLE STATE; STARVATION-SURVIVAL; ENTERIC BACTERIA; MICROBIAL-CELLS; AQUATIC SYSTEMS; FLOW-CYTOMETRY; IN-SITU; IDENTIFICATION; ENVIRONMENT; SEAWATER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Domingo, JWS US EPA, Natl Exposure Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA US EPA 26 W Martin Luther King Dr Cincinnati OH USA 45268 USA
Citazione:
J.W.S. Domingo et al., "Survival of Salmonella species in river water", CURR MICROB, 40(6), 2000, pp. 409-417

Abstract

The survival of four Salmonella strains in river water microcosms was monitored by culturing techniques, direct counts, whale-cell hybridization, scanning electron microscopy, and resuscitation techniques via the direct viable count method and flow cytometry. Plate counts of bacteria resuspended infiltered and untreated river water decreased several orders of magnitude within the first week of incubation, while they did not decrease as rapidly in autoclaved water. In situ hybridization studies suggested a rapid decrease in ribosomal content, as determined by the drastic decrease in the number of detectable cells after 72 h. In contrast, direct counts remained relatively constant during 45 days in all microcosoms. Although the culturable counts of two bacterial strains in filtered water after 31 days represented approximately 0.001% of the total counts, direct viable counts and resuscitation studies with a dilution series suggested that the number of viable bacteria was at least four orders of magnitude higher. Additionally, notable changes in forward scatter and in nucleic acid content were observed only after 4 h of nutrient amendments by flow cytometry. However, cells from the resuscitation experiments did not grow on solid media unless cell-free supernatant from viable cultures was added during the resuscitation period. Theresults in this study suggest the presence of a not immediately culturablestatus in Salmonella.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:28:39