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Titolo:
8(S)-hydroxyeicosatetraenoic acid is the lipoxygenase metabolite of arachidonic acid that regulates epithelial cell migration in the rat cornea
Autore:
Yamada, M; Proia, AD;
Indirizzi:
Keio Univ, Sch Med, Dept Ophthalmol, Shinjuku Ku, Tokyo 160, Japan Keio Univ Tokyo Japan 160 Dept Ophthalmol, Shinjuku Ku, Tokyo 160, Japan Duke Univ, Med Ctr, Dept Ophthalmol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Ctr, Dept Ophthalmol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA Duke Univ Durham NCUSA 27710 Med Ctr, Dept Pathol, Durham, NC 27710 USA
Titolo Testata:
CORNEA
fascicolo: 3, volume: 19, anno: 2000, supplemento:, S
pagine: S13 - S20
SICI:
0277-3740(200005)19:3<S13:8AITLM>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-INFLAMMATORY AGENTS; RABBIT CORNEA; 12(S)-HYDROXYEICOSATETRAENOIC ACID; MOUSE SKIN; 12(R)-HYDROXYEICOSATRIENOIC ACID; SURFACE EXPRESSION; OCULAR-TISSUES; HAIRLESS MOUSE; CYTOCHALASIN-D; BIOSYNTHESIS;
Keywords:
arachidonic acid; cornea; hydroxyeicosatetraenoic acid; lipoxygenase; migration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Yamada, M Keio Univ, Sch Med, Dept Ophthalmol, Shinjuku Ku, 35 Shinanomachi, Tokyo 160, Japan Keio Univ 35 Shinanomachi Tokyo Japan 160 chi, Tokyo 160, Japan
Citazione:
M. Yamada e A.D. Proia, "8(S)-hydroxyeicosatetraenoic acid is the lipoxygenase metabolite of arachidonic acid that regulates epithelial cell migration in the rat cornea", CORNEA, 19(3), 2000, pp. S13-S20

Abstract

Background. We previously found that the inhibition of lipoxygenases resulted in delayed epithelial wound closure in organ-cultured rat corneas. The present study was undertaken to determine the lipoxygenase enzyme and metabolite(s) responsible for regulating reepithelialization and their mechanismof action. Methods. The effects of esculetin-an established lipoxygenase inhibitor-on endogenous hydroxyeicosatetraenoic acids (HETEs) production, epithelial wound closure, filamentous-actin (F-actin) cytoskeleton, and mitotic rate were investigated using a cell-culture assay and an organ-culture assay of rat corneal epithelium. Results. Lipoxygenase inhibition by esculetin, which resulted in the disruption of F-actin organization and a decreasein the mitotic rare, delayed wound closure in both cell- and organ-cultureassays. Normal corneoscleral rims metabolized [H-3]arachidonic acid to 12-HETE (major metabolite), 8-HETE, and 9-HETE. HETE synthesis was inhibited by esculetin in a dose-dependent fashion. Chiral-phase analysis revealed that they contained only (S)-enantiomers, which indicated that they were lipoxygenase metabolites. The inhibitory effects of esculetin on F-actin organization and epithelial wound closure in an organ-culture assay were totally reversed by exogenously added 8(S)-HETE, whereas 12- and 9-HETE had no effect. However, none of the HETEs reversed the decreased mitotic rate or achieved complete wound closure in the cell-culture assay. Conclusions. These results suggest that 8(S)-HETE is the key metabolite of arachidonic acid that regulates corneal epithelial cell migration during wound healing. The metabolite responsible for cell proliferation remains to be determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:02:18