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Titolo:
The frequency and proliferative potential of megakaryocytic colony-formingcells (Meg-CFC) in cord blood, cytokine-mobilized peripheral blood and bone marrow, and their correlation with total CFC numbers: implications for the quantitation of Meg-CFC to predict platelet engraftment following cord blood transplantation
Autore:
von Drygalski, A; Xu, G; Constantinescu, D; Kashiwakura, I; Farley, T; Dobrila, L; Rubinstein, P; Adamson, JW;
Indirizzi:
Blood Ctr SE Wisconsin Inc, Blood Res Inst, Milwaukee, WI 53201 USA Blood Ctr SE Wisconsin Inc Milwaukee WI USA 53201 Milwaukee, WI 53201 USA New York Blood Ctr, Lindsley F Kimball Res Inst, Hematopoiet Growth Factors Lab, New York, NY USA New York Blood Ctr New York NY USA Growth Factors Lab, New York, NY USA New York Blood Ctr, Stem Cell Proc Serv, Valhalla, NY USA New York Blood Ctr Valhalla NY USA Stem Cell Proc Serv, Valhalla, NY USA Lindsley F Kimball Res Inst, Fred Allen Lab Immunogenet, New York, NY USA Lindsley F Kimball Res Inst New York NY USA mmunogenet, New York, NY USA
Titolo Testata:
BONE MARROW TRANSPLANTATION
fascicolo: 10, volume: 25, anno: 2000,
pagine: 1029 - 1034
SICI:
0268-3369(200005)25:10<1029:TFAPPO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
EX-VIVO EXPANSION; HEMATOPOIETIC STEM-CELLS; SERUM-FREE SYSTEM; PROGENITOR CELLS; CD34(+) CELLS; SELF-RENEWAL; G-CSF; HUMAN THROMBOPOIETIN; UNRELATED DONORS; PLACENTAL-BLOOD;
Keywords:
cord blood; platelets; colony-forming cells; proliferative potential;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: von Drygalski, A Blood Ctr SE Wisconsin Inc, Blood Res Inst, POB 2178, Milwaukee, WI 53201 USA Blood Ctr SE Wisconsin Inc POB 2178 Milwaukee WI USA 53201
Citazione:
A. von Drygalski et al., "The frequency and proliferative potential of megakaryocytic colony-formingcells (Meg-CFC) in cord blood, cytokine-mobilized peripheral blood and bone marrow, and their correlation with total CFC numbers: implications for the quantitation of Meg-CFC to predict platelet engraftment following cord blood transplantation", BONE MAR TR, 25(10), 2000, pp. 1029-1034

Abstract

CFC numbers have shown to correlate with success of engraftment and speed of neutrophil recovery following cord blood (CB) transplantation. To investigate whether the number of Meg-CFC in a CB stem cell preparation might correlate with time to platelet engraftment, we evaluated the frequency of Meg-CFC among all CFC types in 134 CB, 21 adult bone marrow (BM) and 52 cytokine-mobilized peripheral blood (PB) stem cell preparations. The correlation of Meg-CFC with the total number of CFC and mixed cell-CFC was also assessed. The frequency of Meg-CFC was highest in CB and correlated significantly with total CFC numbers (mean 20.8%, correlation coefficient (r) 0.84, P=0.0001) compared with Meg-CFC from mobilized PB (mean 13.1%, v = 0.29, P = 0.07) and BM (mean 4%, r = 0.39, P = 0.13), In addition, mixed-cell CFC numbers in CB were highly correlated with the total number of Meg-CFC (r=0.7, P=0.0001). No such correlations were found with mobilized PB or BM, We conclude that, based on the high degree of correlation between Meg-C-FC and non-Meg-CFC numbers in CB. no additional information concerning time to platelet engraftment would be gained by routinely performing Meg-CFC assays in addition to non-Meg-CFC assays, The fact that CB Meg-CFC and mixed-cell CFC are strongly correlated suggests that CB Meg-CFC are more primitive than their counterparts in BM and PB and may reflect the number of stem cells in CB.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 08:46:50