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Titolo:
Development of new ethanologenic Escherichia coli strains for fermentationof lignocellulosic biomass
Autore:
Dien, BS; Nichols, NN; OBryan, PJ; Bothast, RJ;
Indirizzi:
USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biochem Res Unit, Peoria, IL 61604 USA USDA ARS Peoria IL USA 61604 entat Biochem Res Unit, Peoria, IL 61604 USA
Titolo Testata:
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
, volume: 84-6, anno: 2000,
pagine: 181 - 196
SICI:
0273-2289(200021)84-6:<181:DONEEC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTATE-DEHYDROGENASE; ZYMOMONAS-MOBILIS; CORN FIBER; CONVERSION; XYLOSE; SUGARS; KO11; PATHWAY; GENES; YEAST;
Keywords:
alcohol; biofuel; Escherichia coli; fermentation; pentoses;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Dien, BS USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biochem Res Unit, 1815 N Univ St, Peoria, IL 61604 USA USDA ARS 1815 N Univ St Peoria IL USA 61604 Peoria, IL 61604 USA
Citazione:
B.S. Dien et al., "Development of new ethanologenic Escherichia coli strains for fermentationof lignocellulosic biomass", APPL BIOC B, 84-6, 2000, pp. 181-196

Abstract

Two new ethanologenic strains (FBR4 and FBR5) of Escherichia coli were constructed and used to ferment corn fiber hydrolysate. The strains carry the plasmid pLOI297, which contains the genes from Zymomonas mobilis necessary for efficiently converting pyruvate into ethanol. Both strains selectively maintained the plasmid when grown anaerobically. Each culture was serially transferred 10 times in anaerobic culture with sugar-limited medium containing xylose, but no selective antibiotic. An average of 93 and 95% of the FBR4 and FBR5 cells, respectively, maintained pLOI297 in anaerobic culture. The fermentation performances of the repeatedly transferred cultures were compared with those of cultures freshly revived from stock in pH-controlled batch fermentations with 10% (w/v) xylose. Fermentation results were similarfur all the cultures. Fermentations were completed within 60 h and ethanolyields were 86-92% of theoretical. Maximal ethanol concentrations were 3.9-4.2% (w/v). The strains were also tested for their ability to ferment cornfiber hydrolysate, which contained 8.5% (w/v) total sugars (2.0% arabinose, 2.8% glucose, and 3.7% xylose). E. coli FBR5 produced more ethanol than FBR4 from the corn fiber hydrolysate. E. coli FBR5 fermented all but 0.4% (w/v) of the available sugar, whereas strain FBR4 left 1.6% unconsumed. The fermentation with FBR5 was completed within 55 h and yielded 0.46 g of ethanol/g of available sugar, 90% of the maximum obtainable.

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Documento generato il 04/04/20 alle ore 01:45:55