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Titolo:
Chromatographic and immunochemical approaches to the analysis of the HIV protease inhibitor saquinavir in plasma
Autore:
Wiltshire, HR; Wiltshire, BG; Clarke, AF; Worth, E; Prior, KJ; Tjia, JF;
Indirizzi:
Roche Discovery Welwyn, Welwyn Garden City AL7 3AY, Herts, England Roche Discovery Welwyn Welwyn Garden City Herts England AL7 3AY , England Univ Liverpool, Dept Pharmacol & Therapeut, Liverpool L69 3GE, Merseyside,England Univ Liverpool Liverpool Merseyside England L69 3GE E, Merseyside,England
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 1, volume: 281, anno: 2000,
pagine: 105 - 114
SICI:
0003-2697(20000515)281:1<105:CAIATT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ULTRAVIOLET DETECTION;
Keywords:
bioanalysis; HPLC/UV; radioimmunoassay; HIV protease inhibitor; saquinavir;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Wiltshire, HR Roche Discovery Welwyn, 40 Broadwater Rd, Welwyn Garden CityAL7 3AY, Herts, England Roche Discovery Welwyn 40 Broadwater Rd Welwyn Garden City Herts England AL7 3AY
Citazione:
H.R. Wiltshire et al., "Chromatographic and immunochemical approaches to the analysis of the HIV protease inhibitor saquinavir in plasma", ANALYT BIOC, 281(1), 2000, pp. 105-114

Abstract

The development of the HIV protease inhibitor saquinavir (Ro 31-8959) required a range of analytical methods for its measurement in biological fluids. This paper describes the development of isocratic, reverse-phase HPLC/UV methods for the routine measurement of plasma levels of the drug together with a more sensitive radioimmunoassay, The performance of the two assays iscompared with that of an HPLC/MS/MS method previously published and has been shown to be satisfactory, with coefficients of variation of calibration standards and quality control samples within the usual outside limits of +/-15%. The HPLC/UV method can be routinely applied for concentrations down to 10-20 ng/ml and a lower limit of quantification of 1 ng/ml from 1 mi of human plasma is possible. The radioimmunoassay was developed for the specific measurement of saquinavir concentrations in human, HIV-positive plasma samples and has a lower limit of quantification of 0.5-1.0 ng/ml, Some preliminary findings suggested that it might not be specific in rat plasma and noattempts have been made to quantify any nonclinical samples with this technique. If still greater sensitivity is required, recourse can be made to the HPLC/MS/MS assay. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:29:39