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Titolo:
MOLECULAR REASONS FOR LIPASE-SENSITIVITY AGAINST ACETALDEHYDE
Autore:
WEBER HK; ZUEGG J; FABER K; PLEISS J;
Indirizzi:
GRAZ UNIV TECHNOL,INST ORGAN CHEM,STREMAYRGASSE 16 A-8010 GRAZ AUSTRIA GRAZ UNIV TECHNOL,INST ORGAN CHEM A-8010 GRAZ AUSTRIA UNIV STUTTGART,INST TECH BIOCHEM D-70569 STUTTGART GERMANY
Titolo Testata:
Journal of molecular catalysis. B, Enzymatic
fascicolo: 1-4, volume: 3, anno: 1997,
pagine: 131 - 138
SICI:
1381-1177(1997)3:1-4<131:MRFLAA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACYL-TRANSFER-REACTIONS; PROTEINS;
Keywords:
LIPASE DEACTIVATION; PK(A)-VALUE; LYSINE RESIDUE ACCESSIBILITY; SCHIFF BASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
H.K. Weber et al., "MOLECULAR REASONS FOR LIPASE-SENSITIVITY AGAINST ACETALDEHYDE", Journal of molecular catalysis. B, Enzymatic, 3(1-4), 1997, pp. 131-138

Abstract

The molecular reasons for the sensitivity of microbial lipases towards acetaldehyde, emerging as unavoidable by-product from acyl transfer reactions employing vinyl esters as acyl donors, were shown to be associated with specific properties of lysine residues. Since the mechanism of deactivation involves the formation of Schiff bases at the lysineepsilon-amino groups, the relative reactivity (i.e, nucleophilicity) of each residue was estimated by using an electronic (pK(a) value) anda steric parameter (accessible surface area of the side chain). Sensitive lipases, as from Candida rugosa and Geotrichum candidum, possess several lysine residues that have high pK(a) values(> 12) and are highly exposed to the solvent (surface areas of 210-220 Angstrom). In contrast, the lysine groups of stable lipases like from Rhizomucor miehei,Candida antarctica B and Pseudomonas glumae have moderate pK(a) values (up to 11.6) and are rather buried (surface areas of 130-150 Angstrom(2)). A close investigation of Candida rugosa lipase revealed that the most exposed lysine residues are located in the lid region (Lys75 and Lys85). The data suggest that Lys75, which is involved in fixing thelid in its open conformation, is presumably the prime target for deactivation by acetaldehyde.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 17:37:29