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Titolo:
Optical coherence microscopy. A technology for rapid, in vivo, non-destructive visualization of plants and plant cells(1[w])
Autore:
Hettinger, JW; Mattozzi, MD; Myers, WR; Williams, ME; Reeves, A; Parsons, RL; Haskell, RC; Petersen, DC; Wang, R; Medford, JI;
Indirizzi:
Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 iol, Ft Collins, CO 80523 USA Harvey Mudd Coll, Dept Biol, Claremont, CA 91711 USA Harvey Mudd Coll Claremont CA USA 91711 ept Biol, Claremont, CA 91711 USA Harvey Mudd Coll, Dept Phys, Claremont, CA 91711 USA Harvey Mudd Coll Claremont CA USA 91711 ept Phys, Claremont, CA 91711 USA Harvey Mudd Coll, Dept Engn, Claremont, CA 91711 USA Harvey Mudd Coll Claremont CA USA 91711 ept Engn, Claremont, CA 91711 USA
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 1, volume: 123, anno: 2000,
pagine: 3 - 15
SICI:
0032-0889(200005)123:1<3:OCMATF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARABIDOPSIS-THALIANA; LIGHT-SCATTERING; LEAF BLADE; SHOOT APEX; TOMOGRAPHY; CELLS; MERISTEM; ROOT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Medford, JI Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 ins, CO 80523 USA
Citazione:
J.W. Hettinger et al., "Optical coherence microscopy. A technology for rapid, in vivo, non-destructive visualization of plants and plant cells(1[w])", PLANT PHYSL, 123(1), 2000, pp. 3-15

Abstract

We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events in shoot tissues without causing damage. With OCM we canimage cells or groups of cells that are up to 1 mm deep in living tissues,resolving structures less than 5 mu m in size, with a typical collection time of 5 to 6 min. OCM measures the inherent light-scattering properties ofbiological tissues and cells. These optical properties vary and provide endogenous developmental markers. Singly scattered photons from small (e.g. 5x 5 x 10 mu m) volume elements (voxels) are collected, assembled, and quantitatively false-colored to form a three-dimensional image. These images can be cropped or sliced in any plane. Adjusting the colors and opacities assigned to voxels allows us to enhance different features within the tissues and cells. We show that light-scattering properties are the greatest in regions of the Arabidopsis shoot undergoing developmental processes. In large cells, high light scattering is produced from nuclei, intermediate light scatter is produced from cytoplasm, and little if any light scattering originates from the vacuole and cell wall. OCM allows the rapid, repetitive, non-destructive collection of quantitative data about inherent properties of cells, so it provides a means of continuously monitoring plants and plant cells during development and in response to exogenous stimuli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 02:00:28