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Titolo:
The tetraspanin CD63/lamp3 cycles between endocytic and secretory compartments in human endothelial cells
Autore:
Kobayashi, T; Vischer, UM; Rosnoblet, C; Lebrand, C; Lindsay, M; Parton, RG; Kruithof, EKO; Gruenberg, J;
Indirizzi:
Univ Geneva, Dept Biochem, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 Biochem, CH-1211 Geneva 4, Switzerland Univ Geneva, Med Ctr, Dept Clin Biochem, CH-1211 Geneva, Switzerland Univ Geneva Geneva Switzerland CH-1211 chem, CH-1211 Geneva, Switzerland Univ Geneva, Hop Cantonal, Div Angiol & Hemostasis, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 mostasis, CH-1211 Geneva 4, Switzerland Univ Queensland, Dept Physiol & Pharmacol, Ctr Microscopy & Microanal, St Lucia, Qld 4072, Australia Univ Queensland St Lucia Qld Australia 4072 St Lucia, Qld 4072, Australia Univ Queensland, Ctr Cellular & Mol Biol, St Lucia, Qld 4072, Australia Univ Queensland St Lucia Qld Australia 4072 St Lucia, Qld 4072, Australia
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 5, volume: 11, anno: 2000,
pagine: 1829 - 1843
SICI:
1059-1524(200005)11:5<1829:TTCCBE>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
WEIBEL-PALADE BODIES; LYSOSOMAL MEMBRANE-GLYCOPROTEINS; VON-WILLEBRAND-FACTOR; NIEMANN-PICK-DISEASE; P-SELECTIN; INTRACELLULAR TRAFFICKING; MULTIVESICULAR ENDOSOMES; REGULATED SECRETION; CYTOPLASMIC DOMAIN; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Gruenberg, J Univ Geneva, Dept Biochem, CH-1211 Geneva 4, Switzerland UnivGeneva Geneva Switzerland 4 1211 Geneva 4, Switzerland
Citazione:
T. Kobayashi et al., "The tetraspanin CD63/lamp3 cycles between endocytic and secretory compartments in human endothelial cells", MOL BIOL CE, 11(5), 2000, pp. 1829-1843

Abstract

In the present study, we show that in human endothelial cells the tetraspanin CD63/lamp3 distributes predominantly to the internal membranes of multivesicular-multilamellar late endosomes, which contain the unique lipid lysobisphosphatidic acid. Some CD63/lamp3 is also present in Weibel-Palade bodies, the characteristic secretory organelle of these cells. We find that CD63/lamp3 molecules can be transported from late endosomes to Weibel-Palade bodies and thus that CD63/lamp3 cycles between endocytic and biosynthetic compartments; however, movement of CD63/lamp3 is much slower than that of P-selectin, which is known to cycle between plasma membrane and Weibel-Palade bodies. When cells are treated with U18666A, a drug that mimics the Niemann-Pick type C syndrome, both proteins accumulate in late endosomes and fail to reach Weibel-Palade bodies efficiently, suggesting that P-selectin, likeCD63/lamp3, cycles via late endosomes. Our data suggest that CD63/lamp3 partitions preferentially within late endosome internal membranes, thus causing its accumulation, and that this mechanism contributes to CD63/lamp3 retention in late endosomes; however, our data also indicate that the protein can eventually escape from these internal membranes and recycle toward Weibel-Palade bodies to be reused. Our observations thus uncover the existence of a selective trafficking route from late endosomes to Weibel-Palade bodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 02:24:27