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Titolo:
Role of actin and Myo2p in polarized secretion and growth of Saccharomycescerevisiae
Autore:
Karpova, TS; Reck-Peterson, SL; Elkind, NB; Mooseker, MS; Novick, PJ; Cooper, JA;
Indirizzi:
Washington Univ, Dept Cell Biol & Physiol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 l & Physiol, St Louis, MO 63110 USA Yale Univ, Dept Cell Biol, New Haven, CT 06520 USA Yale Univ New Haven CTUSA 06520 Dept Cell Biol, New Haven, CT 06520 USA Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 ular & Dev Biol, New Haven, CT 06520 USA Yale Univ, Dept Pathol, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 iv, Dept Pathol, New Haven, CT 06520 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 5, volume: 11, anno: 2000,
pagine: 1727 - 1737
SICI:
1059-1524(200005)11:5<1727:ROAAMI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-V MYOSIN; BUDDING YEAST; VESICULAR TRANSPORT; CELL POLARITY; MESSENGER-RNA; PROTEIN; LOCALIZATION; CYTOSKELETON; VESICLES; INVOLVEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Cooper, JA Washington Univ, Dept Cell Biol & Physiol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 , St Louis, MO 63110 USA
Citazione:
T.S. Karpova et al., "Role of actin and Myo2p in polarized secretion and growth of Saccharomycescerevisiae", MOL BIOL CE, 11(5), 2000, pp. 1727-1737

Abstract

We examined the role of the actin cytoskeleton in secretion in Saccharomyces cerevisiae with the use of several quantitative assays, including time-lapse video microscopy of cell surface growth in individual living cells. Inlatrunculin, which depolymerizes filamentous actin, cell surface growth was completely depolarized but still occurred, albeit at a reduced level. Thus, filamentous actin is necessary for polarized secretion but not for secretion per se. Consistent with this conclusion, latrunculin caused vesicles to accumulate at random positions throughout the cell. Cortical actin patches cluster at locations that correlate with sites of polarized secretion. However, we found that actin patch polarization is not necessary for polarized secretion because a mutant, bee1 Delta(las17 Delta), which completely lacks actin patch polarization, displayed polarized growth. In contrast, a mutant lacking actin cables, tpm1-2 tpm2 Delta, had a severe defect in polarized growth. The yeast class V myosin Myo2p is hypothesized to mediate polarized secretion. A mutation in the motor domain of Myo2p, myo2-66, caused growth to be depolarized but with only a partial decrease in the level of overall growth. This effect is similar to that of latrunculin, suggesting that Myo2p interacts with filamentous actin. However, inhibition of Myo2p function by expression of its tail domain completely abolished growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:05:02