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Titolo:
Response of Xenopus Cds1 in cell-free extracts to DNA templates with double-stranded ends
Autore:
Guo, ZJ; Dunphy, WG;
Indirizzi:
CALTECH, Howard Hughes Med Inst, Div Biol, Pasadena, CA 91125 USA CALTECHPasadena CA USA 91125 Med Inst, Div Biol, Pasadena, CA 91125 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 5, volume: 11, anno: 2000,
pagine: 1535 - 1546
SICI:
1059-1524(200005)11:5<1535:ROXCIC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DAMAGE CHECKPOINT; POLYMERASE-ALPHA; SCHIZOSACCHAROMYCES-POMBE; PROTEIN-KINASE; CYCLE CHECKPOINTS; PRIMASE ACTIVITY; HUMAN HOMOLOG; EGG EXTRACTS; SIMIAN CELLS; CHK1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Dunphy, WG CALTECH, Howard Hughes Med Inst, Div Biol, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 Div Biol, Pasadena, CA 91125 USA
Citazione:
Z.J. Guo e W.G. Dunphy, "Response of Xenopus Cds1 in cell-free extracts to DNA templates with double-stranded ends", MOL BIOL CE, 11(5), 2000, pp. 1535-1546

Abstract

Although homologues of the yeast checkpoint kinases Cds1 and Chk1 have been identified in various systems, the respective roles of these kinases in the responses to damaged and/or unreplicated DNA in vertebrates have not been delineated precisely. Likewise, it is largely unknown how damaged DNA andunreplicated DNA trigger the pathways that contain these effector kinases. We report that Xenopus Cds1 (Xcds1) is phosphorylated and activated by thepresence of some simple DNA molecules with double-stranded ends in cell-free Xenopus egg extracts. Xcds1 is not affected by ayhidicolin, an agent that induces DNA replication blocks. In contrast, Xenopus Chk1 (Xchk1) responds to DNA replication blocks but not to the presence of double-stranded DNR ends. Immunodepletion of Xcds1 (and/or Xchk1) from egg extracts did not attenuate the cell cycle delay induced by double-stranded DNA ends. These results imply that the cell cycle delay triggered by double-stranded DNA ends either does not involve Xcds1 or uses a factor(s) that can act redundantly with Xcds1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 02:17:08