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Titolo:
A carotenogenic gene cluster from Brevibacterium linens with novel lycopene cyclase genes involved in the synthesis of aromatic carotenoids
Autore:
Krubasik, P; Sandmann, G;
Indirizzi:
Univ Frankfurt, Inst Bot, Biosynth Grp, D-60054 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60054 Grp, D-60054 Frankfurt, Germany
Titolo Testata:
MOLECULAR AND GENERAL GENETICS
fascicolo: 3, volume: 263, anno: 2000,
pagine: 423 - 432
SICI:
0026-8925(200004)263:3<423:ACGCFB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; STRUCTURAL SIMILARITIES; STREPTOMYCES-GRISEUS; MOLECULAR EVOLUTION; CORYNEFORM BACTERIA; BIOSYNTHETIC GENE; CLONING; EXPRESSION; SYNTHASE; COMPLEMENTATION;
Keywords:
Brevibacterium linens; lycopene cyclase; functional complementation; isorenieratene; carotenogenic genes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Sandmann, G Univ Frankfurt, Inst Bot, Biosynth Grp, POB 111932, D-60054 Frankfurt, Germany Univ Frankfurt POB 111932 Frankfurt Germany D-60054 , Germany
Citazione:
P. Krubasik e G. Sandmann, "A carotenogenic gene cluster from Brevibacterium linens with novel lycopene cyclase genes involved in the synthesis of aromatic carotenoids", MOL G GENET, 263(3), 2000, pp. 423-432

Abstract

The carotenogenic (at) gene cluster from Brevibacterium linens, a member of the commercially important group of coryneform bacteria, was cloned and identified. An expression library of B, linens genes was constructed and a fragment of the crt cluster was obtained by functional complementation of a colourless B. flavum mutant, screening transformed cells for production of a yellow pigment. Subsequent screening of a cosmid library resulted in the cloning of the whole crt cluster from B. linens. All genes necessary for the synthesis of the aromatic carotenoid isorenieratene were identified on the basis of sequence homologies. In addition a novel type of lycopene cyclase was identified by complementation of a lycopene-accumulating B. flavum mutant. Two genes, named crtYc and crtYd, which code for polypeptides of 125 and 107 amino acids, respectively, are necessary to convert lycopene to p-carotene. The amino acid sequences of these polypeptides show no similarity to any of the known lycopene cyclases. This is the first example of a carotenoid biosynthetic conversion in which two different gene products are involved, probably forming a heterodimer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 05:14:41