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Titolo:
The migratory response to platelet-derived growth factor of smooth muscle cells isolated from synthetic vascular grafts in a canine model
Autore:
Minion, DJ; Snajdar, RM; van de Kerkhove, MP; van Aalst, JA; Fox, PL; Graham, LM;
Indirizzi:
Case Western Reserve Univ, Dept Surg, Cleveland, OH 44106 USA Case WesternReserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Cleveland Clin Fdn, Lerner Res Inst, Dept Cell Biol, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 l Biol, Cleveland, OH 44195 USA Univ Michigan Hlth Syst, Dept Surg, Ann Arbor, MI USA Univ Michigan Hlth Syst Ann Arbor MI USA t, Dept Surg, Ann Arbor, MI USA Vet Affairs Med Ctr, Ann Arbor, MI USA Vet Affairs Med Ctr Ann Arbor MI USA Affairs Med Ctr, Ann Arbor, MI USA
Titolo Testata:
JOURNAL OF VASCULAR SURGERY
fascicolo: 5, volume: 31, anno: 2000,
pagine: 953 - 959
SICI:
0741-5214(200005)31:5<953:TMRTPG>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-LIKE MOLECULES; CELLULAR PROLIFERATION; SIGNALING PATHWAYS; GENE-EXPRESSION; DACRON GRAFTS; FACTOR-BB; DISTINCT; INJURY; ANGIOPLASTY; ASSOCIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Minion, DJ Univ Kentucky, Med Ctr, 800 Rose St, Lexington, KY 40536 USA Univ Kentucky 800 Rose St Lexington KY USA 40536 , KY 40536 USA
Citazione:
D.J. Minion et al., "The migratory response to platelet-derived growth factor of smooth muscle cells isolated from synthetic vascular grafts in a canine model", J VASC SURG, 31(5), 2000, pp. 953-959

Abstract

Objective: Previous studies on smooth muscle cells (SMCs) harvested from implanted synthetic grafts demonstrate increased production of platelet-derived growth factor (PDGF) hut decreased proliferative response compared withaortic SMCs. The purpose of this study was to determine the migratory response of graft versus aortic SMCs. Method's: Thoracoabdominal grafts were implanted in beagles. The SMCs wereharvested from the graft and infrarenal aorta. Migration was determined with the use of a razor-scrape assay and computerized image analysis. Results: The mean distance migrated and the number of cells that migrated were greater hi graft SMCs at baseline (185 +/- 18 mu m and 108 +/- 17 cells) compared with aortic cells (110 +/- 10 mu m and 42 +/- 5 cells)(P < .05). Baseline differences persisted after treatment with antibodies to PDGF. The addition of PDGF (10 ng/mL) resulted in increased migration in both graft (229 +/- 23 mu m and 146 +/- 20 cells) and aortic SMCs (130 +/- 9 mu m and 70 +/- 5 cells) compared with baseline (P < .05). The relative increase in response to PDGF was similar between the two groups (P = not significant). Conclusions: Graft SMCs differ phenotypically from aortic SMCs; they exhibit increased basal migration that is independent of autocrine stimulation by PDGF. In contrast to their blunted proliferative response, graft SMCs have a similar migratory response to PDGF compared with aortic SMCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:10:01