Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Visualizing and quantifying evoked cortical activity assessed with intrinsic signal imaging
Autore:
Chen-Bee, CH; Polley, DB; Brett-Green, B; Prakash, N; Kwon, MC; Frostig, RD;
Indirizzi:
Univ Calif Irvine, Dept Neurobiol & Behav, Irvine, CA 92697 USA Univ CalifIrvine Irvine CA USA 92697 obiol & Behav, Irvine, CA 92697 USA Univ Calif Irvine, Ctr Neurobiol Learning & Memory, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 ning & Memory, Irvine, CA 92697 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 2, volume: 97, anno: 2000,
pagine: 157 - 173
SICI:
0165-0270(20000415)97:2<157:VAQECA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT BARREL CORTEX; PRIMARY SOMATOSENSORY CORTEX; FUNCTIONAL-ORGANIZATION; RECEPTIVE-FIELDS; CEREBRAL-CORTEX; LAYER-IV; HEMOGLOBIN CONCENTRATION; SENSORY STIMULATION; WHISKER STIMULATION; NEURONAL-ACTIVITY;
Keywords:
optical imaging; intrinsic signal; rat somatosensory cortex; barrel; whisker; vibrissa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Frostig, RD Univ Calif Irvine, Dept Neurobiol & Behav, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 v, Irvine, CA 92697 USA
Citazione:
C.H. Chen-Bee et al., "Visualizing and quantifying evoked cortical activity assessed with intrinsic signal imaging", J NEUROSC M, 97(2), 2000, pp. 157-173

Abstract

Intrinsic signal imaging (ISI) measures changes in light reflectance from the illuminated cortex (intrinsic signals or IS) attributed to various vascular and metabolic sources that, when using illumination in the 600 nm range, appear to co-localize with neuronal activity. Given the multiple sourcescontributing to the collected IS, the common practice of averaging across an extended post-stimulus time epoch before dividing by baseline data typically visualizes evoked IS overlying both the cortical tissue and the large surface blood vessels. In rat PMBSF, the contribution from these vessels are problematic as they do not co-localize with known PMBSF function. Determining a means for quantifying the evoked IS area poses an additional challenge. Here, we describe how exploiting IS collected shortly after stimulus onset (within 1.5 s), which coincides with fast oxygen consumption of active neurons, visualizes evoked IS overlying the cortical tissue without the large surface vessels. We also describe how the use of absolute thresholds combined with a baseline determined from data collected immediately prior to stimulus onset (within 1 s) targets most precisely a specific evoked IS amplitude, a method that should be especially useful when evoked areas are expected to occupy a substantial portion of the total imaged area and/or when peak activity is expected to differ between subjects. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:05:28