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Titolo:
Effect of ferriprotoporphyrin IX and non-heme iron on the Ca2+ pump of intact human red cells
Autore:
Tiffert, T; Lew, VL; Perdomo, D; Ginsburg, H;
Indirizzi:
Univ Cambridge, Physiol Lab, Cambridge CB2 3EG, England Univ Cambridge Cambridge England CB2 3EG Lab, Cambridge CB2 3EG, England Hebrew Univ Jerusalem, Inst Life Sci, Dept Biol Chem, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 -91904 Jerusalem, Israel
Titolo Testata:
JOURNAL OF MEMBRANE BIOLOGY
fascicolo: 2, volume: 175, anno: 2000,
pagine: 107 - 113
SICI:
0022-2631(20000515)175:2<107:EOFIAN>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SICKLE ERYTHROCYTE; INTRACELLULAR CALCIUM; LIPID-PEROXIDATION; NONHEME IRON; BLOOD-CELLS; MEMBRANE; INHIBITION; DEGRADATION; GLUTATHIONE; MECHANISM;
Keywords:
ferriprotoporphyrin IX; heme; non-heme iron; Ca2+-pump; red blood cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Tiffert, T Univ Cambridge, Physiol Lab, Downing St, Cambridge CB2 3EG, England Univ Cambridge Downing St Cambridge England CB2 3EG G, England
Citazione:
T. Tiffert et al., "Effect of ferriprotoporphyrin IX and non-heme iron on the Ca2+ pump of intact human red cells", J MEMBR BIO, 175(2), 2000, pp. 107-113

Abstract

Previous studies have shown that ferriprotoporphyrin IX (FP) and non-hems iron have a marked inhibitory effect on the Ca2+-Mg2+-ATPase activity of isolated red cell membranes, the biochemical counterpart of the plasma membrane Ca2+ pump (PMCA). High levels of membrane-bound FP and non-heme iron have been found in abnormal red cells such as sickle cells and malaria-infected red cells, associated with a reduced lift: span. It was important to establish whether sublytic concentrations of FP and non-heme iron would also inhibit the PMCA in normal red cells, to assess the possible role of these agents in the altered Ca2+ homeostasis of abnormal cells. Active Ca2+ extrusion by the plasma membrane Ca2+ pump was measured in intact red cells that had bren briefly preloaded with Ca2+ by means of the ionophore A23187. The FP and nonheme iron concentrations used in this study were within the range of those applied to the isolated red cell membrane preparations. The results showed that FP caused a marginal inhibition (similar to 20%) of pump-mediated Ca2+ extrusion and that nonheme iron induced a slight stimulation of the Ca2+ efflux (11-20%), in contrast to the marked inhibitory effects on the Ca2+-Mg2+-ATPase of isolated membranes. Thus, EP and non-he me iron are unlikely to play a significant role in the altered Ca2+ homeostasis of abnormal red cells.

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Documento generato il 22/10/20 alle ore 03:30:37