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Titolo:
Expression of CD94/NKG2 subtypes on tumor-infiltrating lymphocytes in primary and metastatic melanoma
Autore:
Vetter, CS; Straten, PT; Terheyden, P; Zeuthen, J; Brocker, EB; Becker, JC;
Indirizzi:
Univ Wurzburg, Dept Dermatol, D-97080 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97080 rmatol, D-97080 Wurzburg, Germany
Titolo Testata:
JOURNAL OF INVESTIGATIVE DERMATOLOGY
fascicolo: 5, volume: 114, anno: 2000,
pagine: 941 - 947
SICI:
0022-202X(200005)114:5<941:EOCSOT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; CYTOLYTIC T-LYMPHOCYTES; CLASS-I MOLECULES; MHC CLASS-I; INHIBITORY RECEPTORS; IMMUNE-RESPONSE; GENE-COMPLEX; NK CELLS; HLA-E; RECOGNITION;
Keywords:
cytotoxic T lymphocyte; natural killer receptor; T cell response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Becker, JC Univ Wurzburg, Dept Dermatol, Josef Schneider Str 2, D-97080 Wurzburg, Germany Univ Wurzburg Josef Schneider Str 2 Wurzburg Germany D-97080 y
Citazione:
C.S. Vetter et al., "Expression of CD94/NKG2 subtypes on tumor-infiltrating lymphocytes in primary and metastatic melanoma", J INVES DER, 114(5), 2000, pp. 941-947

Abstract

Natural killer receptors are expressed both on natural killer populations and subpopulations of T cells, mainly alpha/beta TCR(+)CD8(+) T cells. We have characterized the expression of the C-type lectin natural killer receptor CD94/NKG2 on tumor-infiltrating lymphocytes in primary and metastatic melanoma lesions. By immunohistochemistry, 5-10% of the tumor-infiltrating lymphocytes, both in primary and metastatic lesions, expressed CD94. More than 95% of these CD94(+) cells coexpressed CD8 and the percentage of CD94 expression within the CD8(+) cell population ranged from 5 to 20% with a higher expression in metastatic lesions. CD94/NKG2 exists both in an inhibitory and an activating form; thus, it was necessary to determine whether the inhibitory CD94/NKG2-A/B, the activating CD94/NKG2-C/E, or both were expressedon tumor-infiltrating lymphocytes. Reverse transcription-polymerase chain reaction using specific primers for NKG2-A/B and C/E mRNA revealed the presence of NKG2-C/E in all primary and metastatic lesions. In contrast, the inhibitory NKG2-A/B was only present in 50% of primary tumors whereas 80% of tumor-infiltrating lymphocytes in metastatic lesions expressed these transcripts. In healthy humans, the mean number of inhibitory natural killer receptors is higher than that of activating receptors, but the opposite was true for tumor-infiltrating lymphocytes in melanoma. The reversal of the ratioof inhibitory to activating natural killer receptors among tumor-infiltrating lymphocytes suggests a regulated event due to either specific factors within the tumor microenvironment, preferential homing of T cell subsets, orcertain stages of T cell activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:40:00