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Titolo:
Mapping of quantitative trait loci (QTL) of differential stress gene expression in rat recombinant inbred strains
Autore:
Dumas, P; Sun, YL; Corbeil, G; Tremblay, S; Pausova, Z; Kren, V; Krenova, D; Pravenec, M; Hamet, P; Tremblay, J;
Indirizzi:
Univ Montreal, CHUM Res Ctr, Montreal, PQ, Canada Univ Montreal Montreal PQ Canada eal, CHUM Res Ctr, Montreal, PQ, Canada Charles Univ, Fac Med 1, Inst Biol, Prague, Czech Republic Charles Univ Prague Czech Republic 1, Inst Biol, Prague, Czech Republic Acad Sci Czech Republ, Inst Physiol, Prague, Czech Republic Acad Sci CzechRepubl Prague Czech Republic iol, Prague, Czech Republic
Titolo Testata:
JOURNAL OF HYPERTENSION
fascicolo: 5, volume: 18, anno: 2000,
pagine: 545 - 551
SICI:
0263-6352(200005)18:5<545:MOQTL(>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; SPONTANEOUSLY HYPERTENSIVE RATS; COLD PRESSOR TEST; BLOOD-PRESSURE; TRANSCRIPTION; PREDICTOR; NECROSIS; PROGRAM; HSP70; CELL;
Keywords:
genetic linkage; hsp; hstf; recombinant inbred strain; stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Hamet, P Hotel Dieu CHUM, Ctr Rech, Lab Cellular Biol Hypertens, 3850 Rue St Urbain, Montreal, PQ H2W 1T8, Canada Hotel Dieu CHUM 3850 Rue St Urbain Montreal PQ Canada H2W 1T8 ada
Citazione:
P. Dumas et al., "Mapping of quantitative trait loci (QTL) of differential stress gene expression in rat recombinant inbred strains", J HYPERTENS, 18(5), 2000, pp. 545-551

Abstract

Objective Stress has been shown to be a major environmental contributor tocardiovascular diseases through its effects on blood pressure variability and cardiac function. The cellular stress response is characterized by the expression of specific heat stress genes (hsps), under the transcriptional control of heat shock transcription factors (HSTFs). The levels of hsp mRNAdepend on the severity of the stress, with hstf1 acting as a stress sensor. The aim of this work was to evaluate the genetic contribution of the variability in hsp expression, and to identify its putative quantitative trait loci (QTL). Methods Twenty recombinant inbred rat strains (RIS) were studied. The animals underwent a standardized, identical 1 h immobilization stress in restraint cages, followed by 1 h of rest before sacrifice. Total RNA was extracted from the head, kidneys and adrenals, and the mRNA levels of hsp27, hsp70,hsp84, hsp86 and hsp105 were measured. The strain distribution pattern (SDP) of hsp expression was correlated with that of 475 polymorphic markers distributed throughout the RIS genome. A polymorphism of rat hstf1 in RIS wasused for its mapping in RIS. Results Despite an identical stress being applied to all strains, hsp expression showed up to a 12-fold gradient with little intra-strain variability, indicative of a strong genetic contribution to the trait Heritability ranged from 50 to 77% for most hsp genes in the three target organs. The continuous SDP of stress gene expression indicated the polygenic nature of the trait A common locus on chromosome 7 (at D7Cebrp187s3 marker) was consistently associated with all hsp expression in most of the organs [with a likelihood of odds (LOD) score of 3.0 for hsp27 expression]. We have mapped rat hstf1 on chromosome 7 at the same locus. Finally, the D4Mit19 marker was significantly associated with hsp84 expression in the heart (LOD score of 3.1). Conclusion Two loci were linked with the differential expression of HSPs in response to immobilization stress in target organs of RIS. The chromosome7 locus unveiled for all HSPs could explain up to 42% of the observed interstrain variability of hsp levels in response to stress. We propose hstf1 as a positional candidate at this locus. I Hypertens 2000, 18:545-551 (C) Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 21:11:42