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Titolo:
Validation of the PowerPlex 1.1 (TM) loci for use in human identification
Autore:
Greenspoon, SA; Lytle, PJ; Turek, SA; Rolands, JM; Scarpetta, MA; Carr, CD;
Indirizzi:
Detroit Police Dept, Forens Sci Div, Serol Trace Evidenc Unit, Detroit, MI48201 USA Detroit Police Dept Detroit MI USA 48201 idenc Unit, Detroit, MI48201 USA Natl Legal Labs, Okemos, MI 48864 USA Natl Legal Labs Okemos MI USA 48864Natl Legal Labs, Okemos, MI 48864 USA
Titolo Testata:
JOURNAL OF FORENSIC SCIENCES
fascicolo: 3, volume: 45, anno: 2000,
pagine: 677 - 683
SICI:
0022-1198(200005)45:3<677:VOTP1(>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TANDEM REPEAT LOCI; POLYMERASE CHAIN-REACTION; CAPILLARY ELECTROPHORESIS; AMPLIFICATION; POPULATION; CASEWORK; SAMPLES; SYSTEMS; SETS;
Keywords:
forensic science; DNA typing; short tandem repeat; D16S539; D7S820; D13S317; D5S818; CSF1PO; TPOX; THO1; vWA; microvariant; validation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Greenspoon, SA Detroit Police Dept, Forens Sci Div, Serol Trace Evidenc Unit, 2600 Brust St, Detroit, MI 48201 USA Detroit Police Dept 2600 Brust St Detroit MI USA 48201 USA
Citazione:
S.A. Greenspoon et al., "Validation of the PowerPlex 1.1 (TM) loci for use in human identification", J FOREN SCI, 45(3), 2000, pp. 677-683

Abstract

STR typing is now the favored method of DNA analysis for the purposes of human identification in the forensic community. The Forensic Services Division of the Detroit Police Department has completed its validation of the PowerPlex 1.1(TM) loci (CSF1PO, TPOX, THO1, vWA. D16S539, D7S820, D13S317, andD5S818) for use in forensic casework. Detroit Metro Area Red Grass sampleswere typed from each of five racial/ethnic groups-the Hispanic, Caucasian,African American, Asian, and American Indian populations-and allele and genotype frequencies were calculated. A rare off-ladder variant (9.1 allele at D7S820) was identified among the database samples. A number of validation studies were performed. DNA was extracted from different substrates and typed as expected, except for the DNA extracted from leather (signal absent from the D16S539, D7S820, D13S317. CSF1PO, and TPOX loci) and from dirt (no PCR product generated). The minor contributor in themixture study (250 pg input DNA) was facile to discern. The Concordance study, the variety of fluids from the same individual, and NIST standards studies all produced the expected results. Finally, STR data confirmed previous DNA typing results from adjudicated casework samples.

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Documento generato il 24/11/20 alle ore 21:53:27