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Titolo:
Separation of the enzyme cofactor pyrroloquinoline quinone and three isomeric analogues by capillary electrophoresis with ion-pairing media
Autore:
Smith, AR; Kirchhoff, JR; Zhang, ZP; Tillekeratne, LMV; Hudson, RA;
Indirizzi:
Univ Toledo, Coll Arts & Sci, Dept Chem, Toledo, OH 43606 USA Univ ToledoToledo OH USA 43606 ts & Sci, Dept Chem, Toledo, OH 43606 USA Univ Toledo, Coll Pharm, Dept Med & Biol Chem, Toledo, OH 43606 USA Univ Toledo Toledo OH USA 43606 ept Med & Biol Chem, Toledo, OH 43606 USA Univ Toledo, Coll Arts & Sci, Dept Biol, Toledo, OH 43606 USA Univ ToledoToledo OH USA 43606 ts & Sci, Dept Biol, Toledo, OH 43606 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 876, anno: 2000,
pagine: 193 - 199
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCHEMICAL DETECTION; ZONE-ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; PQQ; COENZYME; QUINOPROTEINS; DEHYDROGENASE; SELECTIVITY; IDENTITY; SAMPLES;
Keywords:
pyrroloquinoline quinone; enzymes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kirchhoff, JR Univ Toledo, Coll Arts & Sci, Dept Chem, 2801 W Bancroft St,Toledo, OH 43606 USA Univ Toledo 2801 W Bancroft St Toledo OH USA 43606 43606 USA
Citazione:
A.R. Smith et al., "Separation of the enzyme cofactor pyrroloquinoline quinone and three isomeric analogues by capillary electrophoresis with ion-pairing media", J CHROMAT A, 876(1-2), 2000, pp. 193-199

Abstract

The enzyme cofactor pyrroloquinoline quinone (PQQ) was successfully separated from three closely related isomeric analogues by capillary electrophoresis with ultraviolet detection. Rapid and efficient separation of all four negatively charged isomers with baseline resolution was achieved by the addition of low concentrations (1-5 mM) of short chain tetraalkylammonium (TAA) salts to the capillary buffer. The TAA cations act as ion-pairing agents and promote differential migration of the isomers with only a minimal reduction in the electroosmotic flow. The effects of the TAA salt concentration and the alkyl chain length were examined. Detection limits of PQQ and its isomers were in the range of 7-15 mu M with mass detection limits of 98-210 fmol. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 07:13:26