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Titolo:
Cross-linking and characterisation of gelatin matrices for biomedical applications
Autore:
Kuijpers, AJ; Engbers, GHM; Krijgsveld, J; Zaat, SAJ; Dankert, J; Feijen, J;
Indirizzi:
Univ Twente, Inst Biomed Technol, Dept Chem Technol, NL-7500 AE Enschede, Netherlands Univ Twente Enschede Netherlands NL-7500 AE 500 AE Enschede, Netherlands Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ Z Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
fascicolo: 3, volume: 11, anno: 2000,
pagine: 225 - 243
SICI:
0920-5063(2000)11:3<225:CACOGM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYESTER ARTERIAL PROSTHESIS; FIBROBLAST GROWTH-FACTOR; LINKED GELATIN; CONTROLLED-RELEASE; DACRON PROSTHESES; COLLAGEN; MICROSPHERES; CARBODIIMIDE; TISSUE; BIOMATERIALS;
Keywords:
cross-linking; gelatin; carbodiimide; hydrogel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Feijen, J Univ Twente, Inst Biomed Technol, Dept Chem Technol, POB 217, NL-7500 AE Enschede, Netherlands Univ Twente POB 217 Enschede Netherlands NL-7500 AE Netherlands
Citazione:
A.J. Kuijpers et al., "Cross-linking and characterisation of gelatin matrices for biomedical applications", J BIOM SC P, 11(3), 2000, pp. 225-243

Abstract

Cross-linking of gelatin A and B with N,N-(3-dimethylaminopropyl)-N'-ethyl-carbodimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was optimised by varying the NHS/EDC molar ratio at constant EDC concentration. Nativeand cross-linked gelatin gels were characterised using the degree of swelling, the number of free amine groups, the phase transition temperature. andtitration of the carboxylic acid residues. The cross-linking reaction was most efficient at a NHS to EDC molar ratio of 0.2. At higher NHS/EDC molar ratios, the reaction of EDC with NHS becomes more pronounced, thereby reducing the effective amount of EDC for cross-linking. Swelling measurements ofcross-linked gelatin gels gave deviating results when no NHS was used, which was explained by heterogeneous localisation of cross-links in the gelatin gel. The incorporation of undesired compounds into the gelatin gels during the cross-linking reaction was not observed. At optimal NHS to EDC molar ratio, gelatin A and B were cross-linked using increasing EDC/COOHgelatin molar ratios. A range of samples varying from very low cross-link density tovery high cross-link density (at high EDC/COOHgelatin) was obtained. Stability of the gels is enhanced with increasing cross-link density, but a minimal cross-link density is required to obtain gelatin gels which art: stableat 40 degrees C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 03:08:36