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Titolo:
Antibody production by molecular farming in plants
Autore:
Fischer, R; Hoffmann, K; Schillberg, S; Emans, N;
Indirizzi:
Rhein Westfal TH Aachen, Inst Biol Bot Mol Genet 1, D-52074 Aachen, Germany Rhein Westfal TH Aachen Aachen Germany D-52074 , D-52074 Aachen, Germany
Titolo Testata:
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
fascicolo: 2, volume: 14, anno: 2000,
pagine: 83 - 92
SICI:
0393-974X(200004/06)14:2<83:APBMFI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC TOBACCO PLANTS; FUNCTIONAL MONOCLONAL-ANTIBODY; FULL-SIZE ANTIBODY; CHAIN FV-ANTIBODY; NICOTIANA-TABACUM; RECOMBINANT ANTIBODIES; TRANSIENT EXPRESSION; STABLE ACCUMULATION; SUSPENSION-CULTURE; PRODUCTION SYSTEMS;
Keywords:
molecular farming; recombinant antibody; transgenic plant; molecular medicine;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Fischer, R Rhein Westfal TH Aachen, Inst Biol Bot Mol Genet 1, Worringerweg 1, D-52074 Aachen, Germany Rhein Westfal TH Aachen Worringerweg 1 AachenGermany D-52074
Citazione:
R. Fischer et al., "Antibody production by molecular farming in plants", J BIOL REG, 14(2), 2000, pp. 83-92

Abstract

"Molecular farming" is the production of pharmaceutical proteins in transgenic plants and has great potential for the production of therapeutic anti-cancer antibodies and recombinant therapeutic proteins. Plants make fully functional recombinant human or animal antibodies. Cultivating transgenic plants on an agricultural scale will produce almost unlimited supplies of recombinant proteins for uses in medicine. Combinatorial library technology is a key tool for the generation and optimisation of therapeutic antibodies ahead of their expression in plants. Optimised antibody expression can be rapidly verified using transient expression assays in plants before creation of transgenic suspension cells or plantlines. Subcellular targeting signals that increase expression levels and optimise protein stability can be identified and exploited using transient expression to create high expresser plant lines. When high expresser lines have been selected, the final step is the development of efficient purification methods to retrieve functional antibody. Antibody production on an industrial scale is then possible using plant suspension cell culture in fermenters, or by the propagation of stably transformed plant lines in the field. Recombinant proteins can be produced eitherin whole plants or in seeds and tubers, which can be used for the long-term storage of both the protein and its production system. The review will discuss these developments and how we are moving toward the molecular farming of therapeutic antibodies becoming an economic and clinical reality.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:20:41