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Titolo:
Characterization of a P-type Na+-ATPase of a facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum
Autore:
Ueno, S; Kaieda, N; Koyama, N;
Indirizzi:
Chiba Univ, Fac Sci, Dept Chem, Inage Ku, Chiba 2638522, Japan Chiba UnivChiba Japan 2638522 Dept Chem, Inage Ku, Chiba 2638522, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 19, volume: 275, anno: 2000,
pagine: 14537 - 14540
SICI:
0021-9258(20000512)275:19<14537:COAPNO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALKALOPHILIC BACILLUS; MEMBRANE-VESICLES; PROTONMOTIVE FORCE; ACID-TRANSPORT; CYTOPLASMIC PH; SODIUM-IONS; PURIFICATION; BACTERIUM; ENERGY; MECHANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Koyama, N Chiba Univ, Fac Sci, Dept Chem, Inage Ku, Chiba 2638522, Japan Chiba Univ Chiba Japan 2638522 Inage Ku, Chiba 2638522, Japan
Citazione:
S. Ueno et al., "Characterization of a P-type Na+-ATPase of a facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum", J BIOL CHEM, 275(19), 2000, pp. 14537-14540

Abstract

A facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum, possesses a P-type Na+-stimulated ATPase in the membrane (Koyama, N. (1999) Curr. Microbiol. 39, 27-30). In this study, we attempted to purify and characterize the enzyme. The ATPase appears to consist of a single polypeptide with an apparent molecular mass of 100 kDa. The enzyme exhibited an optimum pH for activity at around 9. The enzyme was strongly inhibited by vanadate (50%inhibition observed at 3 mu M) and forms an acylphosphate intermediate, suggesting a P-type ATPase. The enzyme, when reconstituted into soybean phospholipid vesicles, exhibited ATP-dependent Na-22(+) uptake, which was completely inhibited by gramicidin. The reconstituted vesicles exhibited a generation of membrane potential (positive, inside). The enzyme is likely to be involved in an electrogenic transport of Na+.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:24:48