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Titolo:
Glycosyl phosphatidylinositol myristoylation in African trypanosomes - Newintermediates in the pathway for fatty acid remodeling
Autore:
Morita, YS; Acosta-Serrano, A; Buxbaum, LU; Englund, PT;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA JohnsHopkins Univ Baltimore MD USA 21205 l Chem, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 19, volume: 275, anno: 2000,
pagine: 14147 - 14154
SICI:
0021-9258(20000512)275:19<14147:GPMIAT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARIANT SURFACE GLYCOPROTEIN; GLYCOSYLPHOSPHATIDYLINOSITOL MEMBRANE ANCHORS; CROSS-REACTING DETERMINANT; BLOOD-STREAM FORMS; PHOSPHOLIPASE-C; DEVELOPMENTAL VARIATION; BRUCEI; BIOSYNTHESIS; GLYCAN; PRECURSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Englund, PT Johns Hopkins Univ, Sch Med, Dept Biol Chem, 725 N Wolfe St, Baltimore, MD21205 USA Johns Hopkins Univ 725 N Wolfe St Baltimore MD USA 21205 5 USA
Citazione:
Y.S. Morita et al., "Glycosyl phosphatidylinositol myristoylation in African trypanosomes - Newintermediates in the pathway for fatty acid remodeling", J BIOL CHEM, 275(19), 2000, pp. 14147-14154

Abstract

Glycosyl phosphatidylinositol (GPI) anchors in the bloodstream form of Trypanosoma brucei are unusual in that their two fatty acids are myristate. The myristates are added in the final stages of GPI biosynthesis in a remodeling reaction. Remodeling occurs first at the sn-2 position of glycerol, involving removal of a longer fatty acid and subsequent attachment of myristate. The second myristate is then incorporated into the sn-l position, but the mechanism has been unclear due to the unavailability of a reliable cell-free system supporting complete remodeling. Here, we first refined the cell-free system (by removing Mn2+ ions), thereby allowing efficient production of the dimyristoylated GPI precursor. Using this improved system, we made three new discoveries concerning the pathway for fatty acid remodeling. First, we discovered a monomyristoylated GPI (known as glycolipid theta') as anintermediate involved in remodeling at the sn-1 position. Second, we foundan alternative pathway for production of glycolipid theta, the first lyse intermediate in remodeling. The alternative pathway involves an inositol-acylated GPI known as glycolipid lyso-C'. Finally, we found that there is significant breakdown of GPIs during remodeling in the cell-free system, and we speculate that this breakdown has a regulatory role in GPI biosynthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:56:39