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Titolo:
DIFFERENCES IN SEROLOGICAL IGA RESPONSES TO RECOMBINANT BACULOVIRUS-DERIVED HUMAN PAPILLOMAVIRUS E2 PROTEIN IN THE NATURAL-HISTORY OF CERVICAL NEOPLASIA
Autore:
ROCHAZAVALETA L; JORDAN D; PEPPER S; CORBITT G; CLARKE F; MAITLAND NJ; SANDERS CM; ARRAND JR; STERN PL; STACEY SN;
Indirizzi:
CHRISTIE HOSP NHS TRUST,PATERSON INST CANC RES,DEPT MOL BIOL,CANC RESCAMPAIGN MANCHESTER M20 9BX LANCS ENGLAND CHRISTIE HOSP NHS TRUST,PATERSON INST CANC RES,DEPT MOL BIOL,CANC RESCAMPAIGN MANCHESTER M20 9BX LANCS ENGLAND CHRISTIE HOSP NHS TRUST,PATERSON INST CANC RES,DEPT IMMUNOL,CANC RES CAMPAIGN MANCHESTER M20 9BX LANCS ENGLAND MANCHESTER ROYAL INFIRM,N W REG VIRUS LAB MANCHESTER M13 9WL LANCS ENGLAND UNIV YORK,DEPT BIOL,CANC RES UNIT YORK YO1 5DD N YORKSHIRE ENGLAND
Titolo Testata:
British Journal of Cancer
fascicolo: 8, volume: 75, anno: 1997,
pagine: 1144 - 1150
SICI:
0007-0920(1997)75:8<1144:DISIRT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM ANTIBODY-RESPONSES; CONFORMATIONAL EPITOPES; ANTIPEPTIDE ANTIBODIES; E7 ONCOPROTEINS; CANCER PATIENTS; TYPE-16 E6; HPV-16 E6; INFECTION; DNA; CARCINOMA;
Keywords:
HUMAN PAPILLOMAVIRUS; SEROLOGY; E2 PROTEIN; CERVICAL NEOPLASIA; BACULOVIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
L. Rochazavaleta et al., "DIFFERENCES IN SEROLOGICAL IGA RESPONSES TO RECOMBINANT BACULOVIRUS-DERIVED HUMAN PAPILLOMAVIRUS E2 PROTEIN IN THE NATURAL-HISTORY OF CERVICAL NEOPLASIA", British Journal of Cancer, 75(8), 1997, pp. 1144-1150

Abstract

Infection with certain types of human papillomavirus (HPV) presents ahigh risk for me subsequent development ai cervical intraepithelial neoplasia (GIN) and cervical carcinoma. Immunological mechanisms are likely to play a role in control of cervical HPV lesions. The HPV E2 protein has roles in virus replication and transcription, and loss of E2 functions may be associated with progression of cervical neoplasia. Accordingly, it is of interest to monitor immune responses to the E2 protein, and previous studies have reported associations between serological reactivity to E2 peptide antigens and cervical neoplasia. In orderto investigate serological responses to native, full-length E2 protein, we expressed HPV-16 E2 proteins with and without an N-terminal polyhistidine tag using the baculovirus system. Purified HPV-16 E2 proteinwas used to develop enzyme-linked immunosorbent assays to detect serological IgG and IgA responses in cervical neoplasia patients and controls. We found that serum IgA levels against the E2 protein were elevated in CIN patients relative to normal control subjects but were not elevated in cervical cancer patients. Moreover, there appeared to be a gradient of response within cervical neoplasia such that the highest antibody levels were seen in lower grades of neoplasia up to CIN a, whereas lower levels were observed in CIN 3 and still lower levels in cervical carcinoma. These findings suggest that the IgA antibody response to E2 may associate with stage and progression in cervical neoplasia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 09:11:02