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Titolo:
Delimiting the use of comparative genomic hybridization in human myeloid neoplastic disorders
Autore:
Gebhart, E; Verdorfer, I; Saul, W; Trautmann, U; Brecevic, L;
Indirizzi:
Univ Erlangen Nurnberg, Inst Humangenet, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 6, volume: 16, anno: 2000,
pagine: 1099 - 1105
SICI:
1019-6439(200006)16:6<1099:DTUOCG>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LYMPHOBLASTIC-LEUKEMIA; MOLECULAR CYTOGENETIC ANALYSIS; COPY NUMBER CHANGES; SOLID TUMORS; GENETIC ABERRATIONS; FISH; TOOL;
Keywords:
comparative genomic hybridization; comparative genomic hybridization; resolving power; genomic imbalances; mosaics; deletions; leukemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Gebhart, E Univ Erlangen Nurnberg, Inst Humangenet, Schwabachanlage 10, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Schwabachanlage 10 ErlangenGermany D-91054
Citazione:
E. Gebhart et al., "Delimiting the use of comparative genomic hybridization in human myeloid neoplastic disorders", INT J ONCOL, 16(6), 2000, pp. 1099-1105

Abstract

Hematopoietic disorders can be used as a suitable tool of additional information on the actual resolving power of comparative genomic hybridization (CGH). Therefore, CGH examination was performed of DNA extracted from 23 acute and 15 chronic myeloproliferative disorders which had just been analyzedusing classical cytogenetic techniques. In nearly all cases CGH analysis was repeated with reversely labeled probes. A Zeiss Axioplan microscope was equipped with the ISIS 3 system for photometric evaluation of the CGH data. A main group was selected of 34 cases showing karyotypic mosaics when routinely diagnosed by classical cytogenetics. The grade of mosaicism was basically determined from the classical cytogenetic analysis and was additionally defined examining target anomalies by I-FISH analysis in 28 of the cases. The second group included 23 cases with deletions, and in 1 case another informative genomic imbalance could be analyzed. Every target anomaly irrespective of its type could be detected in all cases with an affected cell population equalling or exceeding about 25%, but in none was it below 23%. This value was the lowest and was found in a case, with CGH-detected 20q deletion. The smallest deletions of two bands on 20q which could visually be detected by CGH were estimated in the range of 5-7 Mb. CGH was also suitable to detect imbalances which were not clearly detected by routine cytogenetics. Reverse labelling, performed in nearly all cases, confirmed the result ofthe original CGH analysis. These data not only document the readiness and reliability of CGH studies on human leukemia, but also further contribute to a clearer definition of the limits of the resolving power of this technique.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 04:27:42