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Titolo:
H-ras-1 point mutation in malignant peripheral nerve sheath tumors: Polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues
Autore:
Watanabe, T; Sakamoto, A; Tamiya, S; Oda, Y; Masuda, K; Tsuneyoshi, M;
Indirizzi:
Kyushu Univ, Grad Sch Med Sci, Dept Anat Pathol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ, Grad Sch Med Sci, Dept Clin Radiol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Higashi Ku, Fukuoka 8128582, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 6, volume: 5, anno: 2000,
pagine: 605 - 608
SICI:
1107-3756(200006)5:6<605:HPMIMP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
K-RAS GENE; SOFT-TISSUE; TYPE-1 NEUROFIBROMATOSIS; H-RAS; SARCOMA; ONCOGENE; P53; AMPLIFICATION; ACTIVATION; CARCINOMA;
Keywords:
malignant peripheral nerve sheath tumor; H-ras; mutation; polymerase chain reaction-restriction fragment length polymorphisms; sequencing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuneyoshi, M Kyushu Univ, Grad Sch Med Sci, Dept Anat Pathol, Higashi Ku,3-1-1 Maidashi, Fukuoka 8128582, Japan Kyushu Univ 3-1-1 Maidashi FukuokaJapan 8128582 582, Japan
Citazione:
T. Watanabe et al., "H-ras-1 point mutation in malignant peripheral nerve sheath tumors: Polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues", INT J MOL M, 5(6), 2000, pp. 605-608

Abstract

It has been shown that the NF1 (neurofibromatosis type 1) gene encodes a tumor suppressor which inactivates ras proteins. Among malignant mesenchymaltumors, H-ras-1 mutations have been found in malignant fibrous histiocytoma, leiomyosarcoma and embryonal rhabdomyosarcoma. However, studies on H-ras-1 mutation of many cases of malignant peripheral nerve sheath tumors (MPNST) have not been documented. Therefore, we investigated H-ras-1 mutations of MPNST. In 45 cases of MPNSTs of our files, DNA was extracted from the formalin-fixed paraffin-embedded tissue, and the mutations of the H-ras-1 genewere detected by using PCR-RFLP (polymerase chair, reaction-restriction fragment length polymorphisms) method and direct sequencing. We found two cases with H-ras-1 point mutation in MPNST for the first time. Both cases showed the same mutation in codon 13.1 [GGT(Gly) to AGT(Ser) transition]. Interestingly, both cases were associated with NF1. It is possibile that the mutation of the H-ras-1 gene occurred after the mutation of the NF1 gene in the MPNST.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:06:14