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Titolo:
Induction of the activation-related antigen CD69 on human eosinophils by type IIA phospholipase A(2)
Autore:
Urasaki, T; Takasaki, J; Nagasawa, T; Ninomiya, H;
Indirizzi:
Univ Tsukuba, Coll Med Technol, Tsukuba, Ibaraki 3058577, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058577 sukuba, Ibaraki 3058577, Japan Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Tsukuba, Ibaraki, Japan Yamanouchi Pharmaceut Co Ltd Tsukuba Ibaraki Japan ukuba, Ibaraki, Japan Univ Tsukuba, Inst Clin Med, Dept Hematol, Tsukuba, Ibaraki 305, Japan Univ Tsukuba Tsukuba Ibaraki Japan 305 matol, Tsukuba, Ibaraki 305, Japan
Titolo Testata:
INFLAMMATION RESEARCH
fascicolo: 4, volume: 49, anno: 2000,
pagine: 177 - 183
SICI:
1023-3830(200004)49:4<177:IOTAAC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN NEUTROPHILS; MAST-CELLS; IN-VITRO; EXPRESSION; SURFACE; RECEPTOR; RELEASE; DEGRANULATION; GENERATION; CLONING;
Keywords:
eosinophil; type IIA phospholipase A(2) (PLA2-IIA); CD69; lysophospholipids; GM-CSF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ninomiya, H Univ Tsukuba, Coll Med Technol, Tennodai 1-1-1, Tsukuba, Ibaraki 3058577, Japan Univ Tsukuba Tennodai 1-1-1 Tsukuba Ibaraki Japan 3058577apan
Citazione:
T. Urasaki et al., "Induction of the activation-related antigen CD69 on human eosinophils by type IIA phospholipase A(2)", INFLAMM RES, 49(4), 2000, pp. 177-183

Abstract

Objective: High levels of human type IIA phospholipase A(2) (PLA(2)-IIA) have been found in the eosinophil-mediated inflammation sites, although the pathophysiological role of PLA(2)-IIA in the eosinophil activation has remained poorly understood. We investigated the effects of PLA(2)-IIA on eosinophil activation. Methods: Eosinophils were incubated with recombinant human PLA(2)-IIA or other stimuli, and then eosinophil peroxidase (EPO) (by colorimetric assay) and leukotriene C-4 (by enzyme immunoassay) released in the incubation buffer were measured. Expression of CD11b and CD69 on the cell surface was alsomeasured by flow cytometry (by mean fluorescence intensity (MFI)). EPO, LTC4, and CD11b are thought to be markers for early phase activation (occurred in an hour after stimulation), and CD69 is to be a marker for late phase activation (occurred after several hours). Results: While PLA(2)-IIA (5 mu g/ml) did not induce any early phase activation, it induced significant expression of an activation-related antigen, CD69, on human blood eosinophils. The PLA(2)-IIA, when enzymatically inactivated by either p-bromophenacyl bromide or EDTA, lost its effect on the CD69 induction. Similarly to PLA(2)-IIA, several lysophospholipids (1 mu g/ml)also induced CD69 on eosinophils significantly (control, 0.71 +/- 0.11; PLA(2)-IIA, 3.29 +/- 0.37*; lysophosphatidic acid, 2.57 +/- 0.43*; specific MFI +/- S.E.M., n = 4, *indicate p < 0.05 vs. control). Conclusions: PLA(2)-IIA induces CD69 expression on the eosinophils throughits catalytic activity at least partly via the enzymatic products such as several lysophospholipids from the eosinophil membrane phospholipids. PLA(2)-IIA may contribute to the eosinophilic inflammation synergistically with other factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:21:25