Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expression, characterization, and localization of Rab26, a low molecular weight GTP-binding protein, in the rat parotid gland
Autore:
Yoshie, S; Imai, A; Nashida, T; Shimomura, H;
Indirizzi:
Nippon Dent Univ, Sch Dent, Dept Oral Anat, Niigata 9518580, Japan Nippon Dent Univ Niigata Japan 9518580 Oral Anat, Niigata 9518580, Japan Nippon Dent Univ, Sch Dent, Dept Oral Biochem, Niigata 9518580, Japan Nippon Dent Univ Niigata Japan 9518580 l Biochem, Niigata 9518580, Japan
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 4, volume: 113, anno: 2000,
pagine: 259 - 263
SICI:
0948-6143(200004)113:4<259:ECALOR>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SECRETORY GRANULES; CA2+-DEPENDENT EXOCYTOSIS; REGULATED SECRETION; PANCREATIC ACINI; VESICLE DOCKING; CELLS; TRANSPORT; MEMBRANE; PATHWAYS; GTPASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshie, S Nippon Dent Univ, Sch Dent, Dept Oral Anat, 1-8 Hamauracho, Niigata 9518580, Japan Nippon Dent Univ 1-8 Hamauracho Niigata Japan 9518580 80, Japan
Citazione:
S. Yoshie et al., "Expression, characterization, and localization of Rab26, a low molecular weight GTP-binding protein, in the rat parotid gland", HISTOCHEM C, 113(4), 2000, pp. 259-263

Abstract

We investigated the expression of the genes encoding Rab proteins, low molecular weight GTP-binding proteins, in the rat parotid gland by the use of reverse transcription-polymerase chain reaction, and detected cDNAs of Rab3D, Rab4, and Rab26. We further examined the characteristics and localization of Rab26 by western blotting and light and electron microscopic immunocytochemistry. Western blotting using an antibody against the Rab26-specific, C-terminal peptide detected the His-tagged Rab26 protein as a single 27-kDaband. This band also displayed binding to [alpha-P-32]GTP. The fraction containing secretory granule membranes in an acinar cell homogenate was immunostained with the antibody. Supporting this, the immunocytochemical reaction for Rab26 was localized immediately around secretory granules in the acinar cells. The immunostaining disappeared from the acinar cells after treatment of rats with isoproterenol. These findings suggest that Rab26 participates in the regulated secretion of granules and functionally belongs to the Rab3 group.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:18:08