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Titolo:
Characterization of the human EPLIN (Epithelial Protein Lost in Neoplasm) gene reveals distinct promoters for the two EPLIN isoforms
Autore:
Chen, SX; Maul, RS; Kim, HR; Chang, DD;
Indirizzi:
Univ Calif Los Angeles, Sch Med, Div Heme Onc,Jonsson Comprehens Canc Ctr,Dept Med Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Dent Res Inst, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 248, anno: 2000,
pagine: 69 - 76
SICI:
0378-1119(20000502)248:1-2<69:COTHE(>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAMILY; RAC1;
Keywords:
alternative RNA processing; immediate-early gene; serum response element;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Chang, DD Univ Calif Los Angeles, Sch Med, Div Heme Onc,Jonsson ComprehensCanc Ctr,Dept Med Microbiol Immunol & Mol Genet, 10833 Le Conte Ave, Los Angeles, CA 90095 USA Univ Calif Los Angeles 10833 Le Conte Ave Los Angeles CA USA 90095
Citazione:
S.X. Chen et al., "Characterization of the human EPLIN (Epithelial Protein Lost in Neoplasm) gene reveals distinct promoters for the two EPLIN isoforms", GENE, 248(1-2), 2000, pp. 69-76

Abstract

EPLIN is a novel LIM domain protein that co-localizes to the actin stress fibers and focal adhesion plaques. We previously have demonstrated that twoisoforms, the 600 aa EPLIN-alpha and the 759 aa EPLIN-beta, are generated from a single gene. In the majority of human breast and prostate cancer cell lines, the expression of EPLIN-a is significantly reduced, while the expression of EPLIN-beta is either up-regulated or unchanged. To understand thebasis of this differential regulation, we have determined the organizationof the human EPLIN gene. The human EPLIN gene spans > 100 kb and consists of 11 exons. The EPLIN-beta mRNA requires all 11 exons, while the EPLIN-a mRNA requires Exons 4-11. The transcriptional start sites of EPLIN-a were mapped within the third intron by 5' RACE and Si nuclease protection. Similarly, the 5' ends of EPLIN-beta were mapped upstream of Exon 1. The DNA sequences flanking the EPLIN-alpha or EPLIN-beta transcriptional start sites were capable of stimulating the expression of promoter reporter constructs. Interestingly, the endogenous transcription of EPLIN-a, but not EPLIN-beta, could be stimulated by serum, indicating that the expression of two EPLIN isoforms can be independently regulated. A consensus serum response element was present within 100 bp upstream of the transcriptional start sites of EPLIN-alpha. The activity of 0.7 kb EPLIN-alpha promoter reporter construct could be enhanced by activated RhoA, indicating that this serum response element is functional. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:54:06