Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A S/M DNA replication checkpoint prevents nuclear and cytoplasmic events of cell division including centrosomal axis alignment and inhibits activation of cyclin-dependent kinase-like proteins in fucoid zygotes
Autore:
Corellou, F; Bisgrove, SR; Kropf, DL; Meijer, L; Kloareg, B; Bouget, FY;
Indirizzi:
CNRS, UMR1931, F-29680 Roscoff, France CNRS Roscoff France F-29680CNRS, UMR1931, F-29680 Roscoff, France Biol Stn, Lab Goemar, F-29680 Roscoff, France Biol Stn Roscoff France F-29680 Stn, Lab Goemar, F-29680 Roscoff, France Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 Biol, Salt Lake City, UT 84112 USA Biol Stn, Cell Cycle Grp, F-29680 Roscoff, France Biol Stn Roscoff France F-29680 Cell Cycle Grp, F-29680 Roscoff, France
Titolo Testata:
DEVELOPMENT
fascicolo: 8, volume: 127, anno: 2000,
pagine: 1651 - 1660
SICI:
0950-1991(200004)127:8<1651:ASDRCP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUCUS-DISTICHUS FUCALES; XENOPUS EGG EXTRACTS; SACCHAROMYCES-CEREVISIAE; TYROSINE PHOSPHORYLATION; SPINDLE FORMATION; BUDDING YEAST; MITOSIS; PHAEOPHYCEAE; DEPHOSPHORYLATION; FERTILIZATION;
Keywords:
fucoid zygote; S/M checkpoint; aphidicolin; centrosomes; CDK; cdc25; olomoucine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Bouget, FY CNRS, UMR1931, F-29680 Roscoff, France CNRS Roscoff France F-29680 UMR1931, F-29680 Roscoff, France
Citazione:
F. Corellou et al., "A S/M DNA replication checkpoint prevents nuclear and cytoplasmic events of cell division including centrosomal axis alignment and inhibits activation of cyclin-dependent kinase-like proteins in fucoid zygotes", DEVELOPMENT, 127(8), 2000, pp. 1651-1660

Abstract

S/M checkpoints prevent various aspects of cell division when DNA has not been replicated. Such checkpoints are stringent in yeast and animal somaticcells but are usually partial or not present in animal embryos, Because little is known about S/M checkpoints in plant cells and embryos, we have investigated the effect of aphidicolin, a specific inhibitor of DNA polymerases alpha and delta, on cell division and morphogenesis in Fucus and Pelvetiazygotes, Both DNA replication and cell division were inhibited by aphidicolin, indicating the presence, in fucoid zygotes, of a S/M checkpoint. This checkpoint prevents chromatin condensation, spindle formation, centrosomal alignment with the growth axis and cytokinesis but has no effect on germination or rhizoid elongation. This S/M checkpoint also prevents tyrosine dephosphorylation of cyclin-dependent kinase-like proteins at the onset of mitosis, The kinase activity is restored in extracts upon incubation with cdc25A phosphatase, When added in S phase, olomoucine, a specific inhibitor of cyclin-dependent kinases, has similar effects as aphidicolin on cell division although alignment of the centrosomal axis still occurs. We propose a model involving the inactivation of CDK-like proteins to account for the S/M DNA replication checkpoint in fucoid zygotes and embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:23:27