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Titolo:
Viral contamination of embryos cryopreserved in liquid nitrogen
Autore:
Bielanski, A; Nadin-Davis, S; Sapp, T; Lutze-Wallace, C;
Indirizzi:
Agr Canada, Anim Dis Res Inst, Germplasm Ctr Expertise, Nepean, ON K2H 8P9, Canada Agr Canada Nepean ON Canada K2H 8P9 Expertise, Nepean, ON K2H 8P9, Canada Biol Evaluat Lab, Nepean, ON K2H 8P9, Canada Biol Evaluat Lab Nepean ON Canada K2H 8P9 Lab, Nepean, ON K2H 8P9, Canada
Titolo Testata:
CRYOBIOLOGY
fascicolo: 2, volume: 40, anno: 2000,
pagine: 110 - 116
SICI:
0011-2240(200003)40:2<110:VCOECI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO FERTILIZATION; STEM-CELL COMPONENTS; BOVINE EMBRYOS; DIARRHEA VIRUS; VITRIFICATION METHOD; TRANSMISSION; TRYPSIN; OOCYTES;
Keywords:
viral contamination; cryopreservation; embryo; germplasm; vitrification; liquid nitrogen; BVDV; BIV; BHV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Bielanski, A Agr Canada, Anim Dis Res Inst, Germplasm Ctr Expertise, POB 11300, Nepean,ON K2H 8P9, Canada Agr Canada POB 11300 Nepean ON Canada K2H 8P9 K2H 8P9, Canada
Citazione:
A. Bielanski et al., "Viral contamination of embryos cryopreserved in liquid nitrogen", CRYOBIOLOGY, 40(2), 2000, pp. 110-116

Abstract

Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods of cryopreservation which employ ultrarapid freezing orvitrification require direct contact between the freezing medium containing oocytes or embryos and liquid phase nitrogen (LPN). As models for human and animal viral pathogens three bovine viruses, bovine viral diarrhea virus(BVDV), bovine herpesvirus-1 (BHV), and bovine immunodeficiency virus (BIV), were employed to study the potential for their transmission by experimentally contaminated LN to embryos frozen and stored in open freezing containers. Bovine embryos in a mixture of 20% ethylene glycol, 20% ME2SO, and 0.6% sucrose were vitrified in either unsealed standard 0.25 ml or modified open pulled straws or in plastic cryovials and then plunged into contaminatedLPN. After 3-5 weeks of storage in LN, embryos were thawed and sequentially washed and only those with intact ZP were pooled together and tested in batches of three for viral contamination. From this pool of 83 batches, 13 of 61 (21.3%) batches exposed to BVDV and BHV-1 tested positive for viral association while all 22 batches exposed to BIV in unsealed containers testednegative. All control embryos vitrified in sealed cryovials and straws were free from viral contamination. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 09:00:58