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Titolo:
Quantitative structural and biochemical analyses of tight junction dynamics following exposure of epithelial cells to house dust mite allergen Der p 1
Autore:
Wan, H; Winton, HL; Soeller, C; Gruenert, DC; Thompson, PJ; Cannell, MB; Stewart, GA; Garrod, DR; Robinson, C;
Indirizzi:
St George Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, London SW17 0RE,England St George Hosp London England SW17 0RE harmacol, London SW17 0RE,England Univ Calif San Francisco, Dept Lab Med, Inst Cardiovasc Res, Gene Therapy Core Ctr, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Western Australia, Queen Elizabeth II Med Ctr, Dept Med, Nedlands, WA6009, Australia Univ Western Australia Nedlands WA Australia 6009 ands, WA6009, Australia Univ Western Australia, Queen Elizabeth II Med Ctr, Dept Microbiol, Nedlands, WA 6009, Australia Univ Western Australia Nedlands WA Australia 6009 nds, WA 6009, Australia Univ Manchester, Sch Biol Sci, Manchester, Lancs, England Univ ManchesterManchester Lancs England Sci, Manchester, Lancs, England
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 5, volume: 30, anno: 2000,
pagine: 685 - 698
SICI:
0954-7894(200005)30:5<685:QSABAO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DERMATOPHAGOIDES-PTERONYSSINUS; MEMBRANE-PROTEIN; OCCLUDIN; PERMEABILITY; SEQUENCE; DER-P-1; FIBROBLASTS; DISRUPTION; COMPONENT; CLAUDIN-1;
Keywords:
house dust mite; allergen; Der p 1; tight junction; epithelium; asthma; cysteine peptidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, C St George Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, Cranmer Terrace,London SW17 0RE, England St George Hosp Cranmer Terrace LondonEngland SW17 0RE ngland
Citazione:
H. Wan et al., "Quantitative structural and biochemical analyses of tight junction dynamics following exposure of epithelial cells to house dust mite allergen Der p 1", CLIN EXP AL, 30(5), 2000, pp. 685-698

Abstract

Background House dust mite allergen Der p 1 is a cysteine peptidase. Previously, we have suggested that the proteolytic activity of this allergen maycontribute to asthma by damaging the barrier formed by the airways epithelium. Objective The present study applied novel techniques to compare changes inpermeability with quantitative events in tight junctions (TJs) and desmosomes (DMs) of epithelial cells exposed to Der p 1. Methods Confluent monolayers of Madin-Darby canine kidney (MDCK) and 16HBE14o-human bronchial epithelial cells were used as experimental models. Permeability was estimated from mannitol clearance. Digital imaging with quantification of TJs and DMs was achieved by fluorescent antibody staining and 2-photon molecular excitation microscopy (2PMEM). Biochemical changes in TJswere studied by immunoblotting, radiolabelling and immunoprecipitation. Results Der p 1 caused a time-dependent breakage of TJs and reduction in their content of the protein ZO-1. Reduction in ZO-1 immunofluorescence at TJs occurred with a small increase in the amount of diffuse, cytoplasmic immunoreactive ZO-1 staining. Morpho-logical changes in TJs occurred in synchrony with increases in epithelial permeability. DM puncta increased both in size and intensity of staining. Immunoblotting demonstrated that the disruption of TJ morphology was associated with cleavage of ZO-1 and occludin. Cells recovered from allergen exposure by de novo synthesis of occludin. Conclusion Der p 1 could contribute to sensitization and allergic responses by degrading the function of the airway epithelial barrier.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:08:34