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Titolo:
Skin prick test and serological analysis with recombinant group 2 allergens of the dust mites L-destructor and T-putrescentiae
Autore:
Kronqvist, M; Johansson, E; Magnusson, CGM; Olsson, S; Eriksson, TLJ; Gafvelin, G; Van Hage-Hamsten, M;
Indirizzi:
Danderyd Hosp, Dept Med, Stockholm, Sweden Danderyd Hosp Stockholm Sweden nderyd Hosp, Dept Med, Stockholm, Sweden Karolinska Hosp & Inst, Dept Med, Div Clin Immunol & Allergy, Stockholm, Sweden Karolinska Hosp & Inst Stockholm Sweden ol & Allergy, Stockholm, Sweden
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 5, volume: 30, anno: 2000,
pagine: 670 - 676
SICI:
0954-7894(200005)30:5<670:SPTASA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-IGE AUTOANTIBODIES; BIRCH POLLEN ALLERGEN; DERMATOPHAGOIDES-PTERONYSSINUS; LEPIDOGLYPHUS-DESTRUCTOR; TYROPHAGUS-PUTRESCENTIAE; FARMING POPULATION; DIAGNOSTIC-VALUE; GRASS-POLLEN; ACARUS-SIRO; ANTIBODIES;
Keywords:
Lepidoglyphus destructor; Tyrophagus putrescentiae; recombinant allergens; rLep d 2; rTyr p 2; SPT; RAST; IgG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kronqvist, M Karolinska Hosp, Dept Clin Immunol, S-17176 Stockholm, SwedenKarolinska Hosp Stockholm Sweden S-17176 Stockholm, Sweden
Citazione:
M. Kronqvist et al., "Skin prick test and serological analysis with recombinant group 2 allergens of the dust mites L-destructor and T-putrescentiae", CLIN EXP AL, 30(5), 2000, pp. 670-676

Abstract

Background The dust mites Lepidoglyphus destructor and Tyrophagus putrescentiae are important sources of allergen in farming environments. The major allergens of the dust mites L. destructor and T. putrescentiae have been cloned and expressed as recombinant proteins. Objective To evaluate the use of recombinant group 2 allergens of L. destructor (rLep d 2) and T. putrescentiae (rTyr p 2) in skin prick test (SPT), and serological analysis in sensitized and non-sensitized farmers chronically exposed to dust mites. Methods Skin prick test with rLep d 2, rTyr p 2 and the corresponding commercial extracts was performed in 44 farmers sensitized to L. destructor and/or T. putrescentiae, and 38 control farmers. IgE and IgG subclass antibodies to the recombinant allergens were analysed by RAST and ELISA, respectively. Results Out of the 44 subjects positive in SPT to L. destructor and/or T. putrescentiae extract, 26 (59%) displayed a positive SPT to one or the other of the recombinant allergens, whereas 21 (48%) were positive to both. Significant correlations were registered between the sizes of the weals induced by rLep d 2 and rTyr p 2 and the corresponding RAST values (P < 0.001). Amajority of subjects positive in SPT to the recombinant allergens had detectable IgG4 antibodies, and the levels were significantly higher in the dust mite sensitized group than in the controls (P < 0.05). No such differences were found in the IgG1 values (P > 0.05). The results obtained with rLep d 2 and rTyr p 2 correlated relatively well with each other with respect toSPT, RAST and IgG4, suggesting that the allergens have similar or shared IgE epitopes. All the control subjects had a negative SPT and RAST to rLep d2 and rTyr p 2. Conclusion Recombinant group 2 allergens from the dust mite L. destructor and T. putrescentiae represent useful tools for diagnosis of dust mite allergy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:26:37