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Titolo:
Characterization of fission yeast meiotic mutants based on live observation of meiotic prophase nuclear movement
Autore:
Hiraoka, Y; Ding, DQ; Yamamoto, A; Tsutsumi, C; Chikashige, Y;
Indirizzi:
Commun Res Lab, Kansai Adv Res Ctr, Struct Biol Sect, Nishi Ku, Kobe, Hyogo 6512492, Japan Commun Res Lab Kobe Hyogo Japan 6512492 hi Ku, Kobe, Hyogo 6512492, Japan Commun Res Lab, Kansai Adv Res Ctr, CREST Res Project, Nishi Ku, Kobe, Hyogo 6512492, Japan Commun Res Lab Kobe Hyogo Japan 6512492 hi Ku, Kobe, Hyogo 6512492, Japan
Titolo Testata:
CHROMOSOMA
fascicolo: 1-2, volume: 109, anno: 2000,
pagine: 103 - 109
SICI:
0009-5915(200004)109:1-2<103:COFYMM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCHIZOSACCHAROMYCES-POMBE; LIVING CELLS; CHROMOSOMES; RECOMBINATION; MICROTUBULES; TELOMERES; MEIOSIS; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hiraoka, Y Commun Res Lab, Kansai Adv Res Ctr, Struct Biol Sect, Nishi Ku,588-2 Iwaoka,Iwaoka Cho, Kobe, Hyogo 6512492, Japan Commun Res Lab 588-2 Iwaoka,Iwaoka Cho Kobe Hyogo Japan 6512492
Citazione:
Y. Hiraoka et al., "Characterization of fission yeast meiotic mutants based on live observation of meiotic prophase nuclear movement", CHROMOSOMA, 109(1-2), 2000, pp. 103-109

Abstract

We characterized four meiotic mutants of the fission yeast Schizosaccharomyces pombe by live observation of nuclear movement. Nuclei were stained with either the DNA-specific fluorescent dye Hoechst 33342 or jellyfish green fluorescent protein (GFP) fused with the N-terminal portion of DNA polymerase alpha. We first followed nuclear dynamics in wild-type cells to determine the temporal sequence of meiotic events: nuclear fusion in the conjugatedzygote is immediately followed by oscillatory nuclear movements that continue for 146 min; then, after coming to rest, the nucleus remains in the center of the cell for 26 min before the first meiotic division. Next we examined nuclear dynamics in four meiotic mutants: mei1 (also called mat2), mei4, dhc1, and taz1. Mei1 and mei4 both arrest during meiotic prophase; our observations, however, show that the timing of mei1 arrest is quite differentfrom that of mei4: the mei1 mutant arrests after nuclear fusion but beforestarting the oscillatory nuclear movements, while the mei4 mutant arrests after the nucleus has completed the oscillatory movements but before the first meiotic division. We also show examples of the dynamic phenotypes of dhc1 and taz1, both of which complete meiosis but exhibit impaired nuclear movement and reduced frequencies of homologous recombination: the dhc1 mutantexhibits no nuclear movement after nuclear fusion, while the taz1 mutant exhibits severely impaired nuclear movement after nuclear fusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 08:36:38