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Titolo:
Anti-idiotype RNAs that mimic the leucine-rich nuclear export signal and specifically bind to CRM1/exportin 1
Autore:
Hamm, J; Fornerod, M;
Indirizzi:
Univ Dundee, Dept Biochem, Dundee DD1 5EH, Scotland Univ Dundee Dundee Scotland DD1 5EH pt Biochem, Dundee DD1 5EH, Scotland European Mol Biol Lab, D-69117 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69117 117 Heidelberg, Germany
Titolo Testata:
CHEMISTRY & BIOLOGY
fascicolo: 5, volume: 7, anno: 2000,
pagine: 345 - 354
SICI:
1074-5521(200005)7:5<345:ARTMTL>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
REV ACTIVATION DOMAIN; RESISTANT DECOY RNA; LEPTOMYCIN-B; PORE COMPLEX; INVITRO SELECTION; TERNARY COMPLEX; MESSENGER-RNA; EF-TU; CRM1; RECEPTOR;
Keywords:
HIV-1 Rev; NES-recognition domain; nuclear export; RNA mimicry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Hamm, J Univ Dundee, Dept Biochem, Wellcome Trust Bldg,Dow St, Dundee DD1 5EH, Scotland Univ Dundee Wellcome Trust Bldg,Dow St Dundee Scotland DD1 5EH nd
Citazione:
J. Hamm e M. Fornerod, "Anti-idiotype RNAs that mimic the leucine-rich nuclear export signal and specifically bind to CRM1/exportin 1", CHEM BIOL, 7(5), 2000, pp. 345-354

Abstract

Background: Anti-idiotype approaches are based on the assumption that an antibody recognising a ligand can be structurally related to the receptor. Recently we have generated anti-idiotype RNA aptamers designed to mimic the human immunodeficiency virus-1 (HIV-1) Rev nuclear export signal (NES). Nuclear injection of either NES-peptide conjugates or aptamer causes the inhibition of Rev-mediated export. This implied that NES mimics and export substrate might compete for binding to the NES receptor. The mechanism of inhibition, however, is unknown,Results: The interaction between the export aptamer and CRM1 was characterised in vitro. The aptamer binds specifically to CRM1 and this interaction is sensitive to competition by Rev NES-peptide conjugates. The recognition domain of CRM1 has been mapped and includes residues found previously to affect binding of leptomycin B, a fungicide interfering with nuclear export. Conclusions: Inhibition of Rev-mediated export in vivo by export aptamers appears to result from the binding of the aptamers to the NES-recognition domain of CRM1. This observation demonstrates that anti-idiotype RNA can mimic faithfully structural and functional properties of a protein and can be used to map ligand-binding domains of receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:14:24