Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CD 95-independent mechanisms of IL-2 deprivation-induced apoptosis in activated human lymphocytes
Autore:
Hieronymus, T; Blank, N; Gruenke, M; Winkler, S; Haas, JP; Kalden, JR; Lorenz, HM;
Indirizzi:
Univ Erlangen Nurnberg, Dept Med 3, Inst Clin Immunol Rheumatol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Univ Erlangen Nurnberg, Childrens Hosp, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 6, volume: 7, anno: 2000,
pagine: 538 - 547
SICI:
1350-9047(200006)7:6<538:C9MOID>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC LUPUS-ERYTHEMATOSUS; FAS-MEDIATED APOPTOSIS; CONVERTING-ENZYME ICE; T-CELL ACTIVATION; BCL-X; CASPASE FAMILY; IMMUNE-SYSTEM; DEATH; INTERLEUKIN-2; EXPRESSION;
Keywords:
IL-2; CD95/Fas/Apo-1; lymphokine withdrawal; Canale-Smith-syndrome; caspases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Lorenz, HM Univ Erlangen Nurnberg, Dept Med 3, Inst Clin Immunol Rheumatol, Krankenhausstr 12, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Krankenhausstr 12 Erlangen Germany D-91054
Citazione:
T. Hieronymus et al., "CD 95-independent mechanisms of IL-2 deprivation-induced apoptosis in activated human lymphocytes", CELL DEAT D, 7(6), 2000, pp. 538-547

Abstract

Growth factor deprivation-induced apoptosis plays an important role in several cellular systems. However, knowledge of the molecular mechanisms involved are restricted to a few murine models or tumor cell lines. Therefore, we aimed studying signaling pathways leading to apoptosis in activated humanperipheral T cells after IL-2 withdrawal. Lymphoblasts from patients with CD 95 (Fas/APO-1)-deficiency revealed that functional CD95 was not requiredto induce apoptosis after IL-2 withdrawal, Moreover, apoptosis induction in response to various cytotoxic stimuli was found to be mediated in the absence of functional CD95 but was affirmatorily influenced by IL-2 signaling. Immunoblots showed no downregulation of Bcl-2 or Bcl-x(L) and no upregulation of Bar, whereas decreased mitochondrial membrane potential was readily measurable 24 h after cytokine deprivation. Tetrapeptide inhibitors showed limited efficacy in preventing apoptosis whereas the caspase inhibitor zVAD-FMK potently blocked induction of apoptosis, Cleavage of different fluorogenic substrates revealed multiple caspase enzyme activities in lymphoblasts, which were not negatively affected by the fas mutation. Starting at 8 h after IL-2 withdrawal, upregulation of active caspase-3 but not of caspase-8could be detected, Taken together, our data argue for molecular mechanismsof cytokine deprivation-induced apoptosis in activated human lymphocytes independent of CD95.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 01:52:24