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Titolo:
Local injection of pertussis toxin attenuates morphine withdrawal excitation of rat supraoptic nucleus neurones
Autore:
Brown, CH; Johnstone, LE; Murphy, NP; Leng, G; Russell, JA;
Indirizzi:
Univ Edinburgh, Sch Med, Dept Biomed Sci, Edinburgh EH8 9XD, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH8 9XD Midlothian, Scotland
Titolo Testata:
BRAIN RESEARCH BULLETIN
fascicolo: 2, volume: 52, anno: 2000,
pagine: 115 - 121
SICI:
0361-9230(20000515)52:2<115:LIOPTA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOS-LIKE IMMUNOREACTIVITY; LOCUS COERULEUS NEURONS; OXYTOCIN NEURONS; ANESTHETIZED RATS; C-FOS; ELECTRICAL-ACTIVITY; ICV MORPHINE; BRAIN; CHOLECYSTOKININ; DEPENDENCE;
Keywords:
cholecystokinin; dependence; Fos protein; G-protein; opioid; supraoptic nucleus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Brown, CH Univ Edinburgh, Sch Med, Dept Biomed Sci, Hugh Robson Bldg,George Sq, Edinburgh EH8 9XD, Midlothian, Scotland Univ Edinburgh Hugh Robson Bldg,George Sq Edinburgh Midlothian Scotland EH8 9XD
Citazione:
C.H. Brown et al., "Local injection of pertussis toxin attenuates morphine withdrawal excitation of rat supraoptic nucleus neurones", BRAIN RES B, 52(2), 2000, pp. 115-121

Abstract

Morphine inhibits oxytocin neurones via G(i/o)-protein-linked mu-opioid receptors. Following chronic morphine administration oxytocin cells develop dependence, shown by withdrawal excitation after administration of the opioid antagonist, naloxone. Here, inactivation of G(i/o)-proteins by pre-treatment of morphine-dependent rats with pertussis toxin injected into the left supraoptic nucleus reduced withdrawal-induced Fos protein expression withinthe injected nucleus by 41 +/- 10% compared to the contralateral nucleus, indicating that functional G(i/o)-proteins are essential for the development and/or expression of morphine dependence by oxytocin cells in the supraoptic nucleus. In another group of rats, pertussis toxin did not alter the responses to either systemic cholecystokinin administration or systemic hypertonic saline administration, indicating that pertussis toxin does not prevent oxytocin cells from responding to stimuli that are not mediated by G(i/o)-proteins. Finally, pertussis toxin reduced acute morphine inhibition of systemic hypertonic saline-induced Fos protein expression in the supraoptic nucleus, confirming that pertussis toxin effectively inactivates G(i/o)-proteins in the supraoptic nucleus. Thus, the expression of morphine withdrawal excitation by supraoptic ucleus oxytocin cells requires the functional integrity of G(i/o)- proteins within the nucleus. (C) 2000 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:46:42