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Titolo:
Influence of sequence duration and number of electrical pulses upon rabbitoocyte activation and parthenogenetic in vitro development
Autore:
Escriba, MJ; Garcia-Ximenez, F;
Indirizzi:
Univ Politecn Valencia, Dept Ciencia Anim, Lab Reprod, E-46071 Valencia, Spain Univ Politecn Valencia Valencia Spain E-46071 d, E-46071 Valencia, Spain
Titolo Testata:
ANIMAL REPRODUCTION SCIENCE
fascicolo: 1-2, volume: 59, anno: 2000,
pagine: 99 - 107
SICI:
0378-4320(20000428)59:1-2<99:IOSDAN>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE OOCYTES; MOUSE OOCYTES; EGGS; CALCIUM; EMBRYOS; STIMULATION; INVITRO; SPERM; CA2+;
Keywords:
rabbit; oocyte electroactivation; parthenogenetic development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Escriba, MJ Univ Politecn Valencia, Dept Ciencia Anim, Lab Reprod, Camino Vera 14, E-46071 Valencia, Spain Univ Politecn Valencia Camino Vera 14 Valencia Spain E-46071
Citazione:
M.J. Escriba e F. Garcia-Ximenez, "Influence of sequence duration and number of electrical pulses upon rabbitoocyte activation and parthenogenetic in vitro development", ANIM REPROD, 59(1-2), 2000, pp. 99-107

Abstract

Electroactivation of in vivo mature young rabbit oocytes was investigated here. The effects of four or eight electrical pulse treatment over 90, 150 or 270 min upon oocyte activation frequency and type, and even upon their subsequent in vitro development, were studied. The lowest activation frequency was observed after applying four-pulses over 90 min (54%). However, extending four-pulse treatment duration over 150 or 270 min induced more oocytes to activate (from 84% to 100%), as did the eight pulsing treatments (from91% to 97%). With eight pulses, extending treatment duration improved the normal activation rates (from 47% to 76%; P < 0.05). Nevertheless, the haploid morulae and blastocyst rates decreased significantly with extended eight pulsing treatment duration (morulae: from 94% to 41% and blastocysts: from 31% to 0%). (C) 2000 Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 04:46:31