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Titolo:
Plasma proteins modified by tyrosine nitration in acute respiratory distress syndrome
Autore:
Gole, MD; Souza, JM; Choi, I; Hertkorn, C; Malcolm, S; Foust, RF; Finkel, B; Lanken, PN; Ischiropoulos, H;
Indirizzi:
Childrens Hosp Philadelphia, Stokes Res Inst, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Childrens Hosp Philadelphia, Div Neonatol, Dept Pediat, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Univ Penn, Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 & Biophys, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Div Pulm & Crit Care, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Crit Care, Philadelphia, PA 19104 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 5, volume: 278, anno: 2000,
pagine: L961 - L967
SICI:
1040-0605(200005)278:5<L961:PPMBTN>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRONCHOALVEOLAR LAVAGE FLUID; ACUTE LUNG INJURY; NITRIC-OXIDE SYNTHASE; ALPHA-1-PROTEINASE INHIBITOR; OXIDATIVE MODIFICATION; CHEMICAL MODIFICATION; 3-NITROTYROSINE; INACTIVATION; ELASTASE; PEROXYNITRITE;
Keywords:
nitric oxide; superoxide; ceruloplasmin; alpha(1)-protease inhibitor; fibrinogen; oxidative stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Ischiropoulos, H Childrens Hosp Philadelphia, Stokes Res Inst, 416D Abramson Ctr,34th St & Civ Ctr Blvd, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia 416D Abramson Ctr,34th St & Civ Ctr Blvd Philadelphia PA USA 19104
Citazione:
M.D. Gole et al., "Plasma proteins modified by tyrosine nitration in acute respiratory distress syndrome", AM J P-LUNG, 278(5), 2000, pp. L961-L967

Abstract

The present study identifies proteins modified by nitration in the plasma of patients with ongoing acute respiratory distress syndrome (ARDS). The proteins modified by nitration in ARDS were revealed by microsequencing and specific antibody detection to be ceruloplasmin, transferrin, alpha(1)-protease inhibitor, alpha(1)-antichymotrypsin, and beta-chain fibrinogen. Exposure to nitrating agents did not deter the chymotrypsin-inhibiting activity of al-antichymotrypsin. However, the ferroxidase activity of ceruloplasmin and the elastase-inhibiting activity of alpha(1)-protease inhibitor were reduced to 50.3 +/- 1.6 and 60.3 +/- 5.3% of control after exposure to the nitrating agent. In contrast, the rate of interaction of fibrinogen with thrombin was increased to 193.4 +/- 8.5% of the control value after exposure of fibrinogen to nitration. Ferroxidase activity of ceruloplasmin and elastase-inhibiting activity of the alpha(1)-protease inhibitor in the ARDS patients were significantly reduced (by 81 and 44%, respectively), whereas alpha(1)-antichymotrypsin activity was not significantly altered. Posttranslational modifications of plasma proteins mediated by nitrating agents may offer abiochemical explanation for the reported diminished ferroxidase activity, elevated levels of elastase, and fibrin deposits detected in patients with ongoing ARDS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 20:05:56