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Titolo:
Epidermal growth factor upregulates production of supernumerary hair cellsin neonatal rat organ of Corti explants
Autore:
Lefebvre, PP; Malgrange, B; Thiry, M; van de Water, TR; Moonen, G;
Indirizzi:
Univ Liege, Dept Otolaryngol, Liege, Belgium Univ Liege Liege BelgiumUniv Liege, Dept Otolaryngol, Liege, Belgium Univ Liege, Dept Human Physiol & Pathophysiol, Liege, Belgium Univ Liege Liege Belgium t Human Physiol & Pathophysiol, Liege, Belgium Univ Liege, Dept Tissue & Cell Biol, Liege, Belgium Univ Liege Liege Belgium Liege, Dept Tissue & Cell Biol, Liege, Belgium Albert Einstein Coll Med, Dept Otolaryngol, Bronx, NY 10467 USA Albert Einstein Coll Med Bronx NY USA 10467 laryngol, Bronx, NY 10467 USA Albert Einstein Coll Med, Dept Neurosci, Bronx, NY 10467 USA Albert Einstein Coll Med Bronx NY USA 10467 Neurosci, Bronx, NY 10467 USA
Titolo Testata:
ACTA OTO-LARYNGOLOGICA
fascicolo: 2, volume: 120, anno: 2000,
pagine: 142 - 145
SICI:
0001-6489(2000)120:2<142:EGFUPO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
COCHLEA IN-VITRO; RETINOIC ACID; PROLIFERATION; CULTURE;
Keywords:
critical period; cochlea; inner ear;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Lefebvre, PP Serv Physiol Humaine, Pl Delcour 17, BE-4000 Liege, Belgium Serv Physiol Humaine Pl Delcour 17 Liege Belgium BE-4000 ium
Citazione:
P.P. Lefebvre et al., "Epidermal growth factor upregulates production of supernumerary hair cellsin neonatal rat organ of Corti explants", ACT OTO-LAR, 120(2), 2000, pp. 142-145

Abstract

The organ of Corti is highly ordered, with a single ron of inner hair cells and three rows of outer hair cells. The number of hair cells produced wasthought to be limited by the time of their terminal mitosis (i.e. E14 in the mouse). However, exogenous application of retinoic acid has been shown to stimulate the formation of supernumerary hair cells in organ of Corti explants from E13 to E16 mouse embryos. Using late embryonic and neonatal rat organ of Corti explants, we investigated the potential for production of supernumerary hair cells in more mature auditory sensory epithelia. When newborn rat organ of Corti explants were cultured under control conditions, an area of supernumerary hair cells was observed in a segment of organ of Corti that was at the junction between the basal and middle turns. In these areas of supernumerary hair cells the number of hair cells increased per unit of length, but remained constant per surface unit. further demonstrating the supernumerary character of this phenomenon. Organ of Corti explants treated with epidermal growth factor (EGF) showed a 50% increase in the length of the organ of Corti segment containing supernumerary hair cells. Upregulation of supernumerary hair cell formation by EGF was found to start and be maximal at birth (P0) and to disappear by 2 days after birth (P2). Treatmentof EGF stimulated P0 explants with an antimitotic drug, cytosine arabinoside (ARAc), demonstrated that the production of supernumerary hair cells occurred independently of cell division.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 02:14:52